Ba Zhen Soup Bone Marrow Suppression Of Experimental Research Of Mouse Hematopoietic Regulation

Objective: Bazhen Decoction cured syndrome of deficiency of blood and qi in traditional Chinese medicine of clinic, it was a classics famous prescription of nourish the blood and promoting blood flow. In the present study, our aim is to explore the effect of Bazhen Decoction on hematopoietic regulation and its mechanisms and provide the experimental data for the improvement form of medication. The experimental research has original significance.Methods: By using experimental hematology techniques including: Hemopoietic progenitor cell culture, immunohistochemistry, flow cytometry, and reverse transcriptase-polymerase chain reaction (RT-PCR)etc. With anemic mice model resulted from myelosuppression by complexes methods, Effects and mechanisms of extracts from tonifying qi herbs studied systematically on cell, protein and gene levels.Results: within the range of certain dosage or concentration:(1) Bazhen Decoction could promote obviously the increase of the number of bone marrow cell (BMC), the number of white blood cell (WBC), red blood cell (RBC) and hemoglobin (HB). It is also promote evidently the increase of blood platelet (PLT).(2) Bazhen Decoction could promote the entry of BMC into cell cycle, and decrease the cell proportion of G₀/G₁ phase, and increase the cell proportion of S phase and G₂/M phase in myelosuppressed mice. It also could increase the proliferation index (PI) of BMC.(3) Bazhen Decoction could promote the Colony forming unit-erythroid (CFU-E), colonyformingunit granulocytemacrophage (CFU-GM), colony forming unit-megakaryocvte (CFU-Meg). The proliferate effect of on hemopoietic progenitor cell of the middle-dosage of Bazhen Decoction Prescription Granules was best.(4) Bazhen Decoction could promote significantly the expression of Bcl-2 protein, inhibit the expression of Bax protein of BMC in myelosuppressed mice.(5) Bazhen Decoction could promote obviously the expression of erythropoietin EPO mRNA of blood serum in myelosuppressed mice.Conclusions: Within the range of certain dosage or concentration Bazhen Decoction could promote the recovery of hematopoietic function in myelosuppressed mice.Bazhen Decoction could promote the entry of BMC into cell cycle, and decrease the cell proportion of G₀/G₁ phase, and increase the cell proportion of S phase and G₂/M phase in myelosuppressed mice. So it could increase the proliferation index (PI) of BMC. Promote significantly the expression of Bcl-2 protein and inhibit the expression of Bax protein of BMC in myelosuppressed mice. Promote obviously the expression of erythropoietin EPOmRNA of blood serum in myelosuppressed mice.