Health Polygala Saponins And Honey Polygala Gastrointestinal Topical Steroid And Enzyme Activity Mechanism

Objective:Radix Polygalae,can tranquilize the mind,remove phlegm and reduce swelling,as commonly used.Improper use can lead to some toxic reactions,such as guttural stimulus,nausea,distension and allergic reaction.Earlier research found that Radix Polygalae and the toxic substances saponins have toxic effect of injury and inhibition of gastrointestinal movement.Honey-stir-roasted Radix Polygalae is low in toxicity with higher security.This study focus on the effect of gastroenteric target organ hormones,neurotransmitters,ATPase activity and related substances of Crude Radix P,Saponins and Honey-stir-baked Radix P.We also did exploratory studies of the impact of centrums 5-HT,to provide scientific basis for the explanation for the reason why honey-stir-roasting can release the toxicity of the crude Radix P.Method:â‘ determinated SP and SS by RIA,tested enzyme activity of Ca⁺⁺-ATPase by the colorimetric determination of phosphorus,determinated NO by nitroreductase chromatometry,â‘¡determined MDA by TBA,determined enzyme activity of SOD by hydroxylamine,determined PGE₂ by fluorescence pectrophotometriy.â‘¢determined 5-HT by fluorescence pectrophotometriy.Result:â‘ Crude Radix P can remarkably decrease SP and the activity of Ca⁺⁺-ATPase in gaster tissue and it also can remarkably increase NO in gaster tissue.â‘¡Honey-stir-baked Radix P can decrease SS in gaster tissue,but the effect is lower than saponins;MDA in gaster tissue is remarkably lowered in group of Honey-stir-baked Radix P than in group of crude radix P.There is tendency that Honey-stir-baked Radix P can enhance activity of SOD.â‘¢Crude Radix P,Saponins and Honey-stir-baked Radix P can remarkably decrease PGE₂ in gaster-tissue, Saponins has the strongest effection;saponins can remarkably increase 5-HT in brain tissue.Conclusion:â‘ Crude Radix P and saponins remarkably inhibit gastrointestinal motility,It may relate to the decrease of SP and activity of Ca⁺⁺-ATP,increase of NO. Detoxification mechanism to inhibition on gastrointestinal motility by Honey-stir- baked Radix P may get the relations to regulate and stable activities of SP and Ca⁺⁺-ATPase.â‘¡The stimulus and injury to gastric mucosa by Crude Radix P and saponins may relate to decrease SS,PGE₂,strengthening attack to the gaster tissue cell by oxygen free radicals.The detoxification mechanism of Honey-stir-baked Radix P might relate to regulate and stable SS,PGE₂ and enhance the activity of SOD.