| Purpose: In this paper, studies of rat kidney deficiency femoral osteoporosis,kidney, hypothalamus OPG /RANKL-related changes in protein activity, analysis of the differentiation of osteoclasts, regulation and signal transduction to clarify osteoporosis Kidney Disease and Syndrome Molecular biology; Bushen Chinese intervention through regulation of the activity of these proteins for clinical prevention and treatment of osteoporosis in the provision of rational drug use based on scientific experiments.Material and method: Methods Kidney ovariectomized osteoporosis rat model,the application Bushen Tianjing, Zhuanggu Yisui intervention of Chinese herbal compound, used as a standard normal control rats, rat control as a blank, while particles with Gushukang agent, Gaitianli film as a positive control, Bupi Chinese medicine as a cross-referencing. Delivery after 12 weeks, with sterile materials and laboratory testing indicators: using RT-PCR method, Western blotting detection femur, kidney, hypothalamus OPG / RANKL expression of mRNA and protein.Results: 1. RT-PCR assay: femur, kidney, hypothalamus there OPG / RANKL geneexpression: the measured femoral 1/30PG/RANKL, compared with the normal group, sham-operated group OPG indicators no significant difference (P>0.05), model blank Group OPG gene expression femur decreased significantly (P<0.01), drug after 12 weeks compared with the mold space, Bushen Chinese low-dose, high-dose group, Spleen group Gushukang group can be raised to varying degrees of femoral OPG gene expression (P<0.01), which Gaitianli group (P>0.05), no statistical significance; and the normal group, sham-operated group targets RANKL no significant difference (P> 0.05) model of the blank group femur RANKL gene expression decreased significantly (P <0. 01), drug after 12 weeks compared with the mold space, Bushen Chinese low-dose, high-dose group, Bupi group Gaitianli group Gushukang Group femur can be raised to varying degrees OPG gene expression (P<0.01). Measurement of renal OPG / RANKL, and the normal group, sham group no significant difference in OPG indicators (P> 0.05), model of the blank group OPG gene expression in kidney decreased significantly (P <0. 01), after 12 weeks of medication, and model space group compared with low-dose Bushen Chinese medicine, high-dose group, Spleen OPG may increase gene expression (P<0.01), Gaitianli group Gushukang group for statistical results (P> 0.05), no statistical significance; and normal group, sham-operated group targets RANKL no significant difference (P> 0.05), model of the blank group RANKL gene expression in kidney decreased significantly (P <0.01), drug after 12 weeks compared with the mold space, low kidney medicine dose, high-dose group, Bupi group Gaitianli group Gushukang group can be raised to varying degrees RANKL gene expression in kidney (P <0.01), there are significant differences. Hypothalamus measured OPG / RANKL,Compared with the normal group, sham-operated group OPG indicators no significant difference (P> 0. 05), model of the blank group OPG gene expression in the hypothalamus increased significantly (P <0. 01), drug after 12 weeks compared with the mold space, Bushen Chinese low-dose, high-dose group, Bupi group Gaitianli group Gushukang group can be reduced to varying degrees OPG gene expression in the hypothalamus (P <0. 01); with the normal group, sham-operated group targets RANKL no significant differences (P> 0.05), model of the blank group RANKL gene expression in the hypothalamus increased significantly (P <0. 01), drug after 12 weeks compared with the mold space, Bushen Chinese low-dose, high-dose group, Spleen group covered Tianli Group Gushukang group to varying degrees can be reduced RANKL gene expression (P <0. 01).2. Western blotting detection: femur, kidney, hypothalamus there OPG / RANKL protein expression; measured femur on 1/30PG/RANKL, compared with the normal group, sham-operated group OPG indicators no significant difference (P> 0.05), model of the blank group femur OPG protein expression decreased significantly (P <0. 01), drug after 12 weeks compared with the mold space, Bushen Chinese high-dose group, Gaitianli group Gushukang femur may increase the expression of OPG protein (P <0.01), in which low-dose group, Bupi group (P>0.05), no statistical significance; and the normal group, sham-operated group targets RANKL no significant difference (P>0.05) model of the blank group RANKL protein expression decreased significantly (P<0.01), medication after 12 weeks compared with the mold space, Bushen Chinese low-dose, high-dose group, Bupi group Gaitianli group Gushukang group to varying degrees may increase the expression of RANKL protein (P<0.01), significant differences. Measurement of renal OPG / RANKL, and the normal group, sham-operated group OPG indicators no significant difference (P>0.05), model control group of renal expression of OPG protein decreased significantly (P<0.01), after 12 weeks of medication, and model space group, the kidney of low-dose of Chinese medicine, high-dose group, may increase renal Gaitianli OPG protein expression (P<0.01), Gushukang group can also increase the expression of OPG protein (P<0.05), Spleen group (P>0.05); with the normal group, sham-operated group targets RANKL no significant difference (P>0.05), model of the blank group RANKL protein expression decreased significantly (P<0.01), drug after 12 weeks compared with the mode space, Bushen Chinese low-dose, high-dose group, Gaitianli group Gushukang group can be raised to varying degrees of renal expression of RANKL protein (P <0. 01), significant differences, Bupi group (P> 0.05). Hypothalamus measured OPG / RANKL, and the normal group, shara-operated group OPG indicators no significant difference (P> 0.05), model of the blank group OPG protein expression in the hypothalamus increased significantly (P <0.01), after 12 weeks of medication, and modulus space group, the kidney of low-dose of Chinese medicine, high-dose group, Spleen group Gaitianli group Gushukang group can be reduced to varying degrees OPG gene expression in the hypothalamus (P<0.01); with the normal group, sham operation group targets RANKL no significant difference (P>0.05), model of the blank group RANKL gene expression in the hypothalamus increased significantly (P <0. 01), drug after 12 weeks compared with the mold space, Bushen Chinese low-dose and high dose group, Bupi group Gaitianli group Gushukang group can be reduced to varying degrees RANKL gene expression (P <0.01).Conclusion: 1. Ovariectomy in rats extraction method can reproduce the successof kidney deficiency model of osteoporosis.2. Normal rat bone, kidney, hypothalamus in the level of gene and protein expression of OPG / RANKL, normal mice show that bone, kidney, there Hypothalamus OPG / RANKL, its in the regulation of bone metabolism process, plays an important regulatory role.3. Bone and kidney OPG / RANKL gene and protein expression levels and Hypothalamus OPG / RANKL gene and protein expression levels, may be the deficiency of the kidney of osteoporosis, one of the important mechanism.4. Bushen Zhuanggu prescription can increase bone and kidney OPG / RANKL gene and protein expression in the hypothalamus reduced OPG / RANKL gene and protein expression, prevention and treatment Kidney play the role of osteoporosis. |
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