Study On Anaerobe Phosphorus Removal And The Structure Property Of Microbial Community

In the condition of anaerobic envinronment, research the effects of different mud, different Phosphor and the original concentration of P, different Nitrogen and nitrogen-phosphorus ratio, different Carbon and carbon-phosphorus ratio on the anaerobic phosphorus removal through the anaerobic culture medium experiment, and combine to fall structure progress to follow a search to the microorganisms of each impact factor in stabilization time. And results are as follows.The phosphorus removal efficiency is highest when the mud is EGSB anaerobic sludge or ASBR sludge, and it is better to remove P within the culture medium of ASBR sludge than with the EGSB sludge, compared with the shortly simultaneous nitrification and denitrification P removal sludge mixed by pig manure and fowl dung, SBR sludge and the simultaneously biochemical nitrogen and phosphorus removal sludge. Microbial diversity are of great abundance and its similarity is low when they are stably cultured in various mud source, among which the similarity between ASBR and EGSB is the most.It has the best result to remove P when using the dipotassiun hydrogen phosphate, the monopotassiun phosphate is the second, and the sodium hexametaphosphate is the worst of the three. If using the monopotassium phosphate, as the concentration of P goes up, the P removal quantity increases. However, when the total P is more than 50mg/L, the P removal which keeps 20mg/L will not goes up as the concentration increases. Different phosphorus source and various initial concentrations of phosphorus culture media are cultured stably rendering rich microbial diversity, low similarity of phosphorus source culture media samples, and high similarity of concentration of phosphorus culture media samples. Among all experiments of phosphorus source, we found that anaerobic medium using dipotassiun hydrogen phosphate and sodium phosphite as phosphorus resource have the lowest similarity 55.4%, besides, in the test of initial phosphorus concentration, the initial phosphorus concentration is 50mg/L had the medium high similarity with the other initial phosphorus concentration which are more than 70%It has the best results to remove P when using the ammonium chloride as N source, the ammonium acetate is the second, and the sodium nitrate is the worst of the three. If using the ammonium chloride as N source,it has the best results to remove P when the nitrogen-phosphorus ratio is 1.0.Different nitrogen source and initial Nitrogen-Phosphorus ratios are cultured stably rendering rich microbial diversity, low similarity of nitrogen source culture media samples. Among all experiments of nitrogen source, we found that anaerobic medium using ammonium chloride and peptone as nitrogen source have the lowest similarity 44.3%, besides, in the test of nitrogen-phosphorus ratio, the initial nitrogen-phosphorus ratio that are respectively 0.5 and 1.0 have the lowest anaerobic medium similarity which is just 59.7%.It has the best results to remove P when using the methanol as C source, the beer and the glucose is the second, and the sodium acetate is the worst of the three. The different carbon-phosphorus ratio has a big impact on the total phosphorus removal and COD removal in the anaerobe culture system,as the carbon-phosphorus ratio increases, the total phosphorus removal and COD removal in the anaerobe culture system will increase until it reaches certain degree that the removement will decrease as the carbon-phosphorus ratio increases.Five kinds of various carbon sources cultured stably rendering rich microbial diversity, high similarity. Eight kinds of various carbon sources cultured stably rendering rich microbial diversity and the similarity is not high, similarity between the anaerobic culture mediums with the carbon- phosphorus ratio 8,12 and 1.5 is the higher than that of 24,30and 40.As the condition of culture optimizes,in the condition of each best effect factor,the phosphorus removal in the anaerobic culture medium is raising;the microbialpopulation variety is abundant , the similarity is discrepancy as a whole;As the condition of culture optimizes,the microbialpopulation structure is changing gradually,the amount of the microbialpopulation are reducing gradually.Combine the result of routine experiments, the microbialpopulation which band 4 7 12 13 and 17 stands for are dominant microbialpopulation that remove P to produce phosphine in anaerobic condition, which can be used to the follow-up sequencing to identify the population.