Genetic Diversity Analysis Of Wild Auricularia Auricularia-jude Strains In Hei Longjiang Province

Auricularia auricularia is a delicious mushroom with medical value. Heilongjiang Province is a major production base of Auricularia auricularia which are popular to both domestic and foreign. Auricularia auricularia industry has greatly promoted the local economic development.This experiment was carried out mainly on analysis of molecular fingerprint of wild Auricularia auricula strains in Heilongjiang Province of China. The genetic diversity was objectively evaluated and methods of distinguishing different wild strains were found out. Those were the basis of protecting and utilizing germplasm, breeding, property right safeguarding, reinforcing managing of Auricularia auricularia. Thirty wild Auricularia auricula strains were collected from Heilongjiang Province were analysed using Internal transcribed spacer (ITS) and sequence-related amplified polymorphism (SRAP) markers.1) 30 wild Auricularia auricula strains were all collected from Hei longjiang province and Tissue isolation method was used to separate the wild strains. Through multi-step isolation and cultivation experiment to prove the isolated strains were Auricularia auricular-jude .2) The ITS sequence analysis was indicated that that the size of the ITS of all isolates was identical, being 559 bp, the length of the ITS1 region was 167 bp and there were 4 variable sites, the length of ITS2 region was 234 bp and have 12 variable sites. The 5.8S subunit, flanked by the two ITS spacers, was the most conserved region, it was 158 bp and only have 1 variable site. The G+C content of the ITS1 region ranged from 50.3%~ 52.0%, ITS2 region ranged from 45.8%~47.4%, and the ITS region ranged from 47.5%~ 47.8%. Pairwise distances within groups varied from 0~0.013. The average was 0.007.3) 8 pair SRAP primers (em2+me3, em2+me6, em5+me4, em6+me1, em6+me6, em7+me5, em7+me6, em8+me4) selected were used to amplify genomes of 30 strains. A total of 157 bands were amplified, and 122 of which were found to be polymorphic, Phylogenetic tree constructed using the Unweighted Pair-group Method with Arith metic Averages (UPGMA) method distributed the 30 strains into six groups. Clustering analysis indicated a high level of genetic diversity among Auricularia auricular.4) Strain 138 had farther genetic distance compared with the other strains. thus providing a good level of choice for parent selection in further cross-breeding programs.