| This paper used the fruit body of Auricularia auricular as raw materials,studied the extraction methods, determination, functional characteristic and thefluorescein isothiocyanate (FITC) labeling method of the soluble Auriculariaauricular polysaccharide(AAP) and investigated the distribution and rules of labeledAuricularia auricular polysaccharide in gastrointestinal tract. The main results are asfellows:.This paper optimized the extraction process of polysaccharide on alkaliextraction and the cellulose enzyme extraction. The yields of soluble Auriculariaauricular polysaccharide (SAAP) are30.07%and10.06%respectively; meanwhilethe yields of insoluble glial polysaccharide (IAAGP) are27.89%and87.86%respectively. The alkali extraction can extract the SAAP more easily than thecellulose enzyme extraction.This paper was tentatively establishing the quantitative analysis method ofthe SAAP and IAAGP. The determination condition may make a significant impacton the polysaccharide contents of the Auricularia auricular. The fruit body ofAuricularia auricular was consisted of Mannose, Glucosamine, Glucuronic acid,Glucose, Galactose, Arabinose, and Fucose according to the molar ratio0.90:0.02:0.23:1.00(as a benchmark):0.02:0.02:0.02. The content of amino-sugars in the fruitbody and the hydrolysis samples are lower, which are not as expected. The content ofthe amino-sugars in the samples by colorimetric assay is higher than the ones whichwere obtained from high performance liquid chromatography (HPLC) but the valuesobtained from the two method are no significant difference.The preparation processes (water extraction, enzyme extraction, alkalineextraction) make great effects on nutrient compositions moisture, ash, fat, protein,non-starch Polysaccharides, physicochemical properties hydration capacity andadsorption capacity (including oil-holding capacity, cholesterol/bile acid/nitrite ionadsorption capacity))and biological activity DPPH radical scavenging and hypoglycemic capacity. The alkaline treatments can exact more crude protein thanthe other two treatments; the buffer can affect the ash content in the samples. TheSAAPs extracted are all have the antioxidant capacity and the hypoglycemic capacity,the enzyme treatment sample is best.There are some differences between the black fungus fruit body and thedifferent IAAGPs on the nutrient composition, especially the non-starchpolysaccharide (NSP) and the amino-sugar. The alkali treatment IAAGP with thehighest content of the NSP and amino-sugars are2.79times and1.17times of thecontent in the fruit body respectively and the content of the water treatment IAAGPand the enzyme treatment IAAGP are slightly higher than the content of fruit body.The treatments also have a greater impact on IAAGPs physicochemical properties.The hydration (in addition to the oil-holding capacity is a little below the fruit body)and the adsorption capacity of the enzyme treatment IAAGP is the best, followed bythe water treatment IAAGP, they are both better than fruit body, the treatments havea big influence on the cholate adsorption capacity, the sodium cholate adsorptioncapacity of enzyme treatment IAAGP is4.04times of the fruit body under the1mMsodium cholate, while the hydrated capacity and adsorption capacity of the alkalitreatment IAAGP are worse than the fruit body.Labeling the SAAP with the fluorescein isothiocyanate and established thequantitative analysis methods to track SAAP distribution in gastrointestinal tract anddetect the content in the plasma of mice as time variation. The results are as follows:the SAAP can be quickly emptying into the small intestine by stomach (thefluorescent substance can not be detected in the plasma), and not be absorbed in thesmall intestine part. The SAAP has a longer retention time in the large intestine thanin the small intestine. The molecular weight of the labeled polysaccharide is about7.5×10~5, and its fluorescently labeled rate is96.66%... |
PDF Full Text Request