Relationship Between Expression Of Livin And Clinical Significance In Stage Ⅱ～Ⅲ Colorectal Cancer.

Objective:Livin, as a new family member of inhibitors of apoptosis(IAPs), which is specially expressed in tumor cells, is proved to be correlated to the prognosis of many tumors. The objective of this study is to investigate the expression of Livin in colorectal carcinoma and the relationship with clinical pathological factors.Method:Immunohistochemistry(IHC) was applied to examine the expression of Livin in 60 cases of human colorectal carcinoma, all of which were colorectal adenoma, and 25 cases of normal colorectal tissues. Results were compared with clinical pathologic parameter such as the diameter of the tumor, clinical stage, recrudesce, and other clinical pathological factors.Result:â‘ Positive immunostaining with the antibody of Livin was observed in both of nuclear and cytoplasma of the carcinoma. The positive rates of Livin in colorectal carcinoma and normal mucosa specimens were respectively 53.3% and 12.0%, and there was a significant positive correlation between them (P<0.05).â‘¡The positive rates of Livin in poor differentiation and well or moderate differentiation tumors were respectively 71.4% and 47.8%, and there was a significant positive correlation between the two degrees of differentiation.â‘¢With statistical significance (P<0.05), the positive rates of Livin was higher in Stage III colorectal tumor (76.2%) than that in Stageâ…¡(41.0%).â‘£The positive rate of Livin was higher in the patients without recurrence and/or metastasis than that with recurrence or metastasis with statistical significance (100% vs.34.9%, P<0.05).â‘¥The expression of Livin protein had no significant positive correlation with sex, age, site, primary size and pathologic category(P>0.05).â‘¥The 5-year survival rate of the 60 patients of colorectal cancer was 71.7%. With statistical significance (P<0.05), the positive Livin expression patients'5-year survival rate was 56.3%, while the negative ones' was 89.3%. With statistical significance (P=0.002, P=0.047) positive Livin expression and stage III were independent prognostic factors of Stage II and Stage III colorectal cancer analysed by Cox multivariate analysis.Conclution:â‘ The positive rate of Livin in colorectal cancer was higher than that in normal colorectal tissues. Livin plays and important role in the tumorigenesis and development of the cancer.â‘¡The expression of Livin protein had a significant correlation with degree of differentiation, stage and recurrence and/or metastasis. There is no significant correlation with sex, age, site, primary size and pathologic category.â‘¢Positive Livin expression and stage III were independent prognostic factors of Stage II and Stage III colorectal cancer. Livin may become an important indicator to monitor recurrence and metastasis of colorectal cancer after opration, and even as a new target for targeted cancer therapy.