Changes Of The Estrous Cycle, Ovarian Morphology, And Ultrastructure Of Growing Follicles In Amygdale-kindled Seizures Female Rats

To explore changes of estrous cycle and ovarian morphology and ultrastructure of growing follicles in amygdale-kindled seizures female rats.Methods Thirty five SD rats of 8-week-old were randomly divided to Kindling electrodes (n=15), sham-kindled (n=10), and controls (n=10). Kindling electrodes were implanted in the left basolateral amygdala in adult female rats. The rats were kindled by brief, daily, suprathreshold stimulations with a bipolar electrode. The sham-kindled rats only implanted bipolar electrode in the left basolateral amygdala. Controls were normal female rats. Estrous cycles were monitored with daily vaginal smears. Electrographic and behavioral seizures were monitored,.right Ovary tissues were taken after 20 days of kindled in the diestrus. At the same time, sham-kindled subjects and their yoked controls were sacrificed and their right ovaries were taken in their diestrus. Ovarian morphometric analyses were examined. Results Kindled rats lost ovarian periodicity and they displayed significantly weight gained and ovarian increasing(P<0.05). The result of HE staining showed that,compared with control group and shamed-kindled group, kindled female rats had enlarged ovaries with numerous growing follicles, espically many preantral follicles, and the total number of follicles and atretic follicles increased(P<0.05). The ovarian in study group exhibited polycystic changes. Ultrastructure of growing follicles of kindled rats exhibited increase of apoptotic granulose cells, theca cell hyperplasia, and more secretory granules can be found in theca cells, their ovarian exhibited polycystic changes. Conclusions Seizures initiated in amygdala result in polycystic ovary and loss of normal estrus cycle. Seizures initiated in amygdala result in polycystic ovary,growing follicles showed more apoptosis and increase of the secretion in the theca cell hyperplasia .