| Objectives: To assess the main effect on pharyngitis and the acute toxicity of Yanshuyin Effervescent Tablets for further usage.Methods: Model of dimethylbenzene induced ear edema and agar-pill-implantation- induced hyperplasia in mice were used to observe the anti-inflammatory effects of the tablets; Chicken red blood cells induced mice delayed-type hypersensitivity experiment was used to observe its effect of anti-allergic; Mice trachea phenol red excretion experimental method was used to observe the role of expelling phlegm; minimal inhibitory concentration and minimal bactericidal concentration of Yanshuyin to beta-hemolytic streptococcus, diplococcus pneumoniae , staphylococcus aureus, E.coli, pseudomonas aeruginosa, five commonly susceptible bacteria in vitro were also observed. The mice with the largest concentration and the largest volume of oral administration of Yanshuyin reduced their activities, ate less and gave thin faeces or even diarrhea, but two or three days later after stoping ig they returned to the normal. Seven days later, no death of the animals was found, the average body weight of mice had no significant difference compared with the normal group, and there were no other toxic reactions.Conclusion: Yanshuyin have little toxicity, it has a good anti-inflammatory, anti-allergic, anti-bacteria effect and some expelling phlegm pharmacological effects. It is suitable for the treatment of pharyngitis. Objectives: Establish a new kind of EID model induced by COH, which can really close to the clinical, easy to operate and for drug screening, and explored its mechanisms. Then observed the effect of improving implantation of Bangdeyu on EID mice.Methods: Following the clinical method, treated with HCG 48h after PMSG treatment. Using 5IU, 7.5IU, 10IU dose of PMSG and HCG, observe the implantation rate and number of average implanted blastocyst spot to decide a best method and dose for the model. The doses decided, explored the probable mechanisms of the EID induced by COH. Using HE stain view the endometrium morphological change on day 4 to 6 of pregnancy; radioimmunoassay method test the contents of estrogens and progestin in mice serum; immunohistochemical method observe the expression of ER, PR, NF-κB, CD31 of endometrium; use evans blue dying view the vasopermeability of the endometrium. Using the EID model induced by COH, on the day of impregnation ig low, medium, high does 6 g·kg-1,12 g·kg-1,24 g·kg-1, till the day 8 of pregnancy, to observe the improving effect of different does Bangdeyun on implantation.Results: In different doses of 5IU,7.5IU,10IU PMSG and HCG, the 10IU doses which injected in different days could both reduce the implantation rate and average implanted blastocyst spot, thus chose it for the EID model dose induced by COH. The mechanisms of EID by this COH maybe: 1) COH affects the concentration of estrogens and progestin in mice serum and their receptors. Compared with normal mice serum concentration of estrogens and progestin peaked at day 4 of pregnancy, COH group peaked at day 5 of pregnancy, ratio of estrogens/progestin, normal mice peaked at day 6 of pregnancy, COH mice peaked at day 7, ER expression was delayed in time and space, weaked or lack, but PR had no obvious change; 2) COH delayed endometrium decidualization; 3) COH may interfere the mother's immune tolerance to blastocyst, mainly manifested NF-κB expressed higher at day 6 of pregnancy than in normal group, NF-κB is a nuclear transcription fator which induce inflammatory factor express and transcription; 4) COH may affect the early placenta angiogenesis. It manifested mainly the evans blue dying of mice endometrium lighter on day 4 to 6 of pregnancy in COH group than in normal group, which means COH may affect the vascular permeability; and in endometrium of normal mice vessels, CD31 began highly expressed around the blastocyst, but COH mice expressed lower. Bangdeyun in 6 g·kg-1 to 24 g·kg-1 doses all showed obvious promoting effect on embryo implantation.Conclusion: 10IU does PMSG and HCG inject in different day can be used for inducing EID model in COH method, which is close to the clinical, easy to operate and can be replicated well. The mechanisms of EID model induced by COH may associated with leading to estrogens/progestin ratio out of control, endometrium decidualization delayed, interfering the mother's immune tolerance to blastocyst and affecting the early placenta angiogenesis. Bangdeyun can enhance the mice implantation rate of EID induced by COH. |
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