| Objective To explore the neuroglobin (NGB)expression in diabetic rats retina. In order to better understanding the NGB role in the maintenance of retinal nerve cells in diabetic retinopathy.Methods 80 SD rats were randomly divided into 2 groups: 10 rats for the control group, while 70 rats for the observed diabetic group. The diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ); while the control group were just accepted intraperitoneal injection of citrate buffer solution. Immunohistochemistry were used to detect the expression of NGB in retina of each group after the SD rats were put to death.Results NGB protein was mainly found in plexiform layers of retina and ellipsoid region of photoreceptor inner segmen of rats in control group, expression of NGB was weak in other retinal layers, and its intense does not change with the age of rats.While for the observed group, There was a small increase of the NGB intense in the rats retina at 2 weeks, then a considerable increase at 4 weeks, Peaked at 6 weeks,then stabilized at 8 weeks. The expression of NGB mainly in plexiform layers of retina and ellipsoid region of photoreceptor inner segme.Conclusion 1.There is a considerable increasing expression of NGB in diabetic group compare to control group in the diabetic rats retina. This imply that NGB may benefit the oxygen supply to the retinal cells in the condition of diabetes, and may lessen the injury of hypoxia in the diabetic retina.2. The NGB intense increased gradually with course of the diabetic mellitus, then tend to be stabilization, may imply that the saturation of the NGB expression. |
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