The Experiment Of High-dose Vitamins E Supplements To Rat Antioxidant Capacity And Cell Function

Objective:The aim of this article is to through added different amounts of vitamin E to investigate high dose vitamins E antioxidant and the effect of the cell function.Methods:Divided 50 wistar rats into the Control, VE1, VE2, VE3 VE4 groups randomly. Through intragastric administration give different dosage of vitamin E to five groups. The test period is 8 weeks. After death animal and took all the blood, then prepare the red cell membrane. The analysis of oxidation resistance include the avtivities of SOD, GSH-Px, and level of MDA and the cell function were examined. DNA damnification were tested by comet assay. The analysis of cell function include peripheral blood lymphocyte(PPL) fractional conversion dating. Lymphocyte fractional conversion analysis by MTT assay.Results:The result of antioxidant capacity show that dose of 50mg/kg, Contrast with the controls, Rats plasma of VE enhanced 75.6% and the activity of SOD enhanced 14.9% and the activity of GSH-Px (P＜0.05, P＜0.01) was enhanced 8.4% and 51.1%, Contrast with the controls, the level of MDA decreased significantly 57%and 47.2% respectively. When the dose of H2O210μmol/h, group 50mg/kgVE could reduce the DNA oxidative damage (P＜0.05). Contrast with the controls,200mg/kg and 750mg/kg VE could enhance 95.8% and 117.5%.And the activity of SOD decreased significantly. But the activity of GSH-Px and the content of MDA changed not significantly. Contrast with group 50mg/kgVE, and the level of SOD enhanced significantly (P<0.05) GSH-Px and the content of MDA enhanced significantly. The analysis of DNA oxidative damage showed that group 200mg/kg VE and group 750mg/kg VE could aggravate DNA oxidative damage (P<0.01, P<0.01).In conclusion, high dose VE could aggravate DNA oxidative damage and organism hereditary stability decreased. The analysis of cell function showed that VE (50 mg/kg) could enhance lymphocyte(PPL) fractional conversion dating and the red cell membrane liquidity. Contrast with the controls, Supply 200 mg/kg VE and 750 mg/kg VE 8weeks make the lymphocyte(PPL) fractional conversion dating decreased significantly (p<0.05).And the red cell membrane liquidity not changed significantly. Conclusion:50mg/kg VE could enhance the activity of SOD and GSH-Px, while reduce the level of MDA. And VE could reduce the level of oxidative damage, and enhance the red cell membrane liquidity and lymphocyte(PPL) fractional conversion dating. 200mg/kgVE and 750mg/kgVE could reduce the activity of SOD, H2O2 could aggravate oxidative damage.750mg/kgVE could reduce the lymphocyte(PPL) fractional conversion dating. While GSH-Px, MDA and the red cell membrane liquidity not changed significantly.In conclusion, VE (50mg/kg) could enhance the rat oxidation resistance and change the cell function. But high dose VE (200mg/kg,750mg/kg) could decrease the organism oxidation resistance, and as increase as the dose of VE could aggravate oxidative damage.