Complanatus, Alisma Comparative Study Of The Lipid-lowering Effects

Hyperlipidemia (HLP) is a high level of serum total cholesterol (TC) or tirglyceride (TG) and(or) low levels of high density lipoprotein cholesterol (HDL-C). Evidence-Based Medicine studyfound that hyperlipidemia is caused by an independent risk factor for cardiovascular andcerebrovascular diseases. Due to differences in diet, environment and other aspects of foreignhypercholesterolemia more common, more study of cholesterol metabolism, tirglyceridemetabolism, the relative lack of About90million people in China suffering rfom hyperlipidemia,elevated triglyceirdes are relatively common, so lowering tirglyceirdes is increasingly becomingone of the keys of the lipid-loweirng drugs. Therefore, emphasis on research on triglyceirdes isnecessary and valuable. Western medicine lipid-loweirng drugs such as statins can causerhabdomyolysis, ifbrates can lead to liver damage, elevated aminotransferases, and niacin causehigh uric acid, although Western medicine with better lipid-loweirng effects, but also show someside effects the advantages of Chinese medicine, it should carry forward the multi-target,multi-component, multi-channel, the overall regulation of blood lipid metabolism.1, Literature ReviewRetrieval treatment of hyperlipidemia literature published between2001-2011, found thattraditional medicine hyperlipidemia pathogenesis, clinical manifestations, complications, and thatthe disease exists in the Chinese medicine "phlegm","stasis" wet muddy "," obesity "", Vertigo "",stroke "", palpitations "", Chest "Syndrome, pathogenesis, and more oriented vacuity and excessmainly of phlegm, blood stasis block the blood, the virtual primary responsibility of the spleen andkidney dysfunction or deficiency, also found a number of clinical investigations and physical,spleen and kidney yang and phlegm fat lipids in pathogenesis, especially with age, spleen kidneyyang deifciency increased the proportion, and therefore more to BHD drugs and the dampnessdrug use in the treatment of hyperlipidemia. Yang drugs with lipid-loweirng effect of: dodder seed,antler, Epimedium, Eucommia, cinnamon, psoralen, Rosa laevigata, complanatus, Cistanche andother liapid-loweirng effects of dampness medicine: Alism, Fori a capillaris, Plantago, Com Silk,Polygonum, etc..Found in the study of modem medical understanding of hyperlipidemia, tirglyceride lipase (ofATGL) main hydrolysis substrate is a triglyceride, the key rate-limiting enzyme for triglyceirdehydrolysis. ATGL plays an important role in the hyperlipidemia, diabetes, obesity, non-alcoholicfatty liver and other diseases. ATGL discovery of lipid metabolism research has opened up a newpath, to further improve the understanding of the process of lipolysis, the impact of drugs on theactivity of ATGL may regulate lipid metabolism in a new direction.Ideal hyperlipidemia animal models is to promote research and development of hyperlipidemia important factors, rfom the perspective of exogenous and endogenous lipid metabolism pathwaysof feeding method to establish a rat model of hyperlipidemia, found that rats strain, sex, feedformulation, such as cholesterol, lard, sugar, whether it contains anti-thyroid hormone drugs,modeling time, feeding practices and other factors can affect blood lipid levels of rats.2, the expeirmental studyMethods: through the establishment of high-fat diet induced rat model of hyperlipidemia atfersuccessful modeling, model group, serum TC levels were randomly divided into model group, thepositive control group, complanatus high-dose, medium dose, low dose group, Alisma high-dose,medium dose, low dose group. Atfer grouping, the model group and control groups according to1Oml/kg given sterile distilled water, positive drug group lOOmg/kg fenoifbrate complanatus highdose, dose, low-dose group were administered amount of136mg/kg68mg/kg,34mg/kg, theamount of crude drug4g/kg,2g/kg, Ig/kg, giving complanatus ethanol extract of Alisma high dose,medium dose, low dose group were administered dose of98mg/kg,49mg/kg,24.5mg/kg, theamount of crude drug2.64g/kg,1.32g/kg,0.66g/kg, giving Alisma terpens extract.Results:(1)8weeks atfer the high-fat diet-induced model group, serum LDL and TC contentincreased (p <0.05), HDL levels decreased signiifcantly (p <0.05).(2) atfer8weeks of drugtreatment, and compared to model group, the fenoifbrate group, complanatus each dose group,Alisma each dose group are able to reduce serum LDL levels (p less than0.05), complanatus, themiddle dose group, Alisma, low-dose group can reduce serum TC levels (p less than0.05), andother traditional Chinese medicine group must reduce serum TC levels, complanatus high dosescan increase serum HDL levels (p <0.05).(3) The biopsy revealed that fenofibrate with a certainliver injury, but can be significantly reduced hepatic steatosis, improved liver morphology, reduceinflammation and infiltration, and other traditional Chinese medicine group also different degreesof reduction in hepatic steatosis, and reduce and reduce fat vacuoles, improve liver morphology,reducing the role of inflammatory infiltration of the liver cells.Found in the liver lipid content in the detection expeirment, high-fat diet-induced rat model ofhyperlipidemia, and rat liver triglyceride and cholesterol levels compared with blank control groupwas signiifcantly increased (p <0.05), there is a signiifcant lipid accumulation phenomenon, ratherthan the fenofibrate group, complanatus each dose group, Alisma each dose group can effectivelyreduce hyperlipidemia rat model of liver tirglyceride and cholesterol content (p less than0.05),with obvious reduce the role of the liver lipid accumulation.ATGL and blank group, model group to reduce the level found in the liver ATGL and DGAT2content,, detection (p <0.05), DGAT2levels (p <0.05); compared with model group, fenoifbratemay increase the liver ATGL expression (p <0.05) lowered liver DGAT2expression (p <0.05); 6沙苑子泻降脂作用的比较研究,泽compared with model group, the rats of vairous traditional Chinese medicine liver ATGL levelthere was no difference (p>0.05); complanatus high, medium and low-dose group, Alisma highschool dose group, can be lowered liver DGAT2expression (p <0.05).Conclusion: The high-fat diet can increase expression of the rat liver DGAT2and reduced ATGLexpression.2complanatus, Alisma certain lipid-lowering effect, and can reduce liver lipiddeposition, and improve hepatic steatosis.3complanatus each dose group, Alisma, the middle dosegroup can be reduced DGAT2expression, reducing tirglyceirde synthesis, but not in liver ATGLexpression. Fenofibrate has a better lipid-lowering effect, and can reduce the deposition of liverlipids significantly reduce hepatic steatosis. Fenofibrate to reduce liver lipid deposition mechanismmay increase the ATGL expression also reduced DGAT2expression.