Eczema The Nanometer Cream Anti-itch Anti-histamine The Release Of The Role Of Experimental Research

Objective:This study was aimed eczema nano-cream three different concentrations of topical skin antipruritic effect in mice and the concentration of histamine in the skin homogenate tissue. Come to the antipruritic effect of the strongest inhibition of histamine release from the optimal drug concentration, and lay the foundation for further clinical application.Research process and methods1.The antipruritic experiment(4-aminopyridine induced itch experiment) SD experiment,60mice were randomly divided into eczema high concentration of nano-cream group, the group of concentration, low concentration group, the positive control group, model group,blank group(n=10).Using8%sodium sulfide, the mice were back hair removal, with compound dexamethasone acetate cream eczema nano-cream according to the high school three different concentrations evenly coating The back of the hair removal region in mice,twice daily, model group and the blankcoated with the same amount of distilled water.And so forth7days in the last administration,40minutes after subcutaneous injection of0.02%of4-aminopyridine solution in the applicator parts, the number of licking back of mice were observed within10minutes, compare the number of each group of mice licking calculated antipruriticThe inhibitory rate to determine the antipruritic effect. 2.4-aminopyridine (4-AP), the impact of skin histamine concentration in sensitized mice sensitized mice skin histamine concentrationMouse short-necked in the above-mentioned anti-itch after the completion of the experiment were killed,Clipping the same area of the injection site sensitized skin.Frozen in liquid nitrogen made from the skin tissue homogenate total protein concentration, Enzyme-linked immunosorbent assay (ELISA) kits for the determination of the concentrations of histamine Each group were compared histamine concentration to observe the influence of various experimental drugs on the histamine concentration.Results:eczema nano-cream three different concentrations could improve the itch threshold of4-aminopyridine sensitized mouse model, reducing the release of histamine in the skin tissue homogenates, including itching and reduce the high concentration group histamine release the strongest effect and the positive control group showed no statistical significance.Conclusion:eczema cream nano antipruritic effect of the allergic skin of mice can reduce the concentrations of histamine release in allergic skin, with good anti-itch anti-allergy effect.