The BMSCs Joint BFGF Treatment By Core Decompression And Transplantation Of Rabbit Early Hormonal Necrosis Of Femoral Head

ObjectiveDouble injection of endotoxin to rabbit gluteal in the three injections of methylprednisolone steroid-induced femoral head necrosis in early time. The core decompression and implantation of FS as a carrier of bone marrow mesenchymal stem cells (BMSCs) and basic fibroblast growth factor (bFGF). To invest of intervention effect observed with fibrin sealant (FS) as the carrier of BMSCs and bFGF by early core decompression and transplantation in the treatment of rabbit femoral head necrosis, and to explore its mechanism.MethodEighty healthy adult New Zealand white rabbits, the weight is2.6±0.4kg, male and female. By ear vein injection of LPS, dose is10μg/kg weight, a total of two injections, two injection interval of24h. After the second injection of LPS, immediately gluteal injection of methylprednisolone three times, the dose of40mg/kg body weight. Each injection interval is24h. Including the combined effects of toxins and hormones, modeling, four weeks after a total of17New Zealand white rabbits were killed,63remaining.Randomly selected60of them, a random number table, the rabbits were randomly divided into four groups of15each. Group A:the model group (control group) received no treatment; Group B:(core decompression group) by the C-arm X-ray bone needle line femoral marrow core decompression,two on each side of the femoral head core decompression channel; Group C:(bone marrow mesenchymal stem cell transplantation group) model underwent core decompression, there are two on each side of the femoral head core decompression channel,through the core decompression channel transplantation of FS as a carrier of bone marrow mesenchymal stem cells;Group D:(BMSCs bFGF transplant treatment group) after modeling, core decompression,there are two on each side of the femoral head core decompression channel, through the core decompression channel transplantation of FS as a carrier of bone marrow mesenchymal stem cells and basic fibroblast growth factor. Rabbits were sacrificed respectively after the model of8weeks,12weeks,16weeks,femoral head, respectively, gross observation, conventional histopathology slices light. Modeling after eight weeks,12weeks and16weeks, underwent bilateral femoral head1.5T magnetic resonance imaging (MRI) detection to observe the signal changes in femoral head necrosis.In8weeks,12weeks and16weeks, vascular endothelial growth factor, core binding factor immunohistochemical staining,in8weeks,12weeks and16weeks of real-time fluorescent quantitative detection of the expression of VEGF and CbfalmRNA.The above data base on SPSS17.0statistical software for statistical analysis.ResultFemoral head gross specimen to watch,modeling eight weeks A, B, femoral head cartilage surface darker, showing a white; Group C femoral head cartilage surface morphology is rough, articular cartilage surface color micro darken; Group D of the femoral head cartilage surface morphology is smooth and complete,articular cartilage surface color is slightly darker. Group A visible bone marrow cavity necrosis area compared with the previous16weeks further increased,B, C the bone marrow cavity necrosis reduce the area and a small number of new bone formation; Group D seen a lot of new bone formation.Line MRI to detect visible,8weeks, group A necrotic area signal change, expansion of the necrotic area,no significant different in group B, necrotic areas, group C necrosis area has been reduced, group D necrotic area compared with group C further reduced.12and16weeks of necrosis between Group A MRI signal changes, B and C were necrotic areas of the signal decreases, necrosis Signal Group D was close to normal. Pathology studies have shown that,A, B, C and D groups, showing that the group D of the femoral head osteonecrosis area than the other three groups significantly reduced.The number of fat cells is significantly reduced in the bone marrow cavity, trabecular bone density increased,trabecular bone surrounded by a large number of new bone formation, capillary proliferation more, empty bone lacuna rate reduction,bone tissue within VEGF, Cbfal expression was significantly enhanced.Real time fluorescent quantitative detection,at8weeks, A, B, C,D VEGF,CbfalmRNA four groups was no significant difference in the expression,but the model after12and16weeks, C B, D VEGF, CbfalmRNA three expression is increased in different degrees, in which D group had obvious change,The D group at sixteenth weeks and compared to the other three groups, and B, C groups had statistically significant differences (P<0.05), compared with A group with significant difference (P<0.01).Conclusion1. BMSCs compound Fs after core decompression channel can effective graft into the femoral head,in the femoral head micro environment into bone cell differentiation, differentiation of osteoblasts,Osteonecrosis of the femoral head regeneration plays a promoting role.2. BFGF can effectively promote the BMSCs into bone cells, osteoblast differentiation, more effective treatment of early avascular necrosis of the femoral head, to provide the basis for clinical trials.