Research On Treatment Of Aniline Wastewater By Bioaugmentation Agents

Bioaugmentation agents (biological agents) has its unique advantages for the treatmentof municipal sewage, it can quickly complete the biochemical system startup under adverseconditions, improve the removal rate of organic significantly, reduce the generation of solidmaterial, and strengthen nitrification. In the wastewater treatment process, it is easy to add inbacteria directly, but the bacteria are also easily missing, or being swallowed by othermicroorganisms. Mycelium pellet is a kind of bio-granule that can from spontaneously in theprocess of ferment. It has a good biological activity, fast sedimentation velocity, and no needfor carrier, good condition of liquid solid separation. It can be used in the treatment ofindustrial wastewater. Using the mycelium pellet as a carrier, adopting the “mixed bacteriaself-immobilization technology”, to establish a micro-ecology environment made up of mixedbacteria group, in order to let all kinds of immobilized microorganisms play its role together,will become a focus in the field of wastewater treatment.Immobilized strain JH-9with simultaneity culture and adsorption respectively, withmycelium pellet forming by aspergillus Y3as the biomass carrier. The simultaneity culturemycelial pellets showed the rapid degradation rate of aniline because of that fixed more strainJH-9at the same time period and collection of the mycelial pellets’ adsorption capacity andhighly active degradation ability of strains JH-9.When compared with the mycelial pelletsfixed only a mixture of aniline-degrading bacteria and the mycelial pellets fixed a mixture ofaniline-degrading bacteria as well as flocculation bacteria,the mycelial pellets fixed with theCOD fast-degrading bacteria as well as a mixture of aniline-degrading bacteria has moreabvious advantages.Use the mycelial pellets as a biomass carrier in the mixed-scale study of SBR, the reactorachieved the stability of the degradation of aniline, and the reactor with the carrier of mycelialpellets has the fast settling velocity, clear Supernatant. PCR-DGGE analysis showed that thefunctional flora on the mycelial pellets, especially aniline-degrading bacteria, grow in a largenumber,and it has higher number of bacteria per unit volume, in favor of bioaugmentation andquick Startup.Add the nano-tourmaline to the reactor with a carrier of mixed mycelial pellets, themycelial pellets showed excellent degradation result of COD and aniline in the first cycle.Onthe second cycle, the aniline removal rate of output was100%and the COD removal rateabove85%.Thus, it shorten the mixing time for the domestication of mycelial pellets, and the reactor achieved a quick startup. PCR-DGGE study shows that the addition of tourmalinefeatures conducive to the rapid stabilization of the functional flora, improve the effectivenessof bioaugmentation.