| BST-2/CD317/HM1.24/tetherin is a B-cell antigen overexpressed on the surfaceof myeloma cell lines and on neoplastic plasma cells of patients with multiplemyeloma. BST-2restricts the secretion of retroviruses, including humanimmunodeficiency virus type1, as well as members of the herpesvirus, filovirus, andarenavirus families, presumably by tethering nascent virions to the cell surface.Theexact cellular function of human BST-2(hBST-2) in the human host is unclear, it hasbeen shown to play a role in regulating the growth and development of B cells, to beinvolved in the organization of the subapical actin cytoskeleton in polarized epithelialcells.In our experiment, BST2gene was cloned, and the recombinant BST2proteinexpressed. After nickel-affinity chromatography and gel filtration chromatography,BST2proteins with a purity of over99%. Monoclonal antibodies (MAbs) againstBST2were produced. Spleen cells collected from BALB/c mice immunized with thewhole cell antigen of BST2were fused with murine Sp2/0myeloma cells.2hybridoma cell lines specific to BST2was established after screening and subcloning.McAbs were obtained，its species specific epitope located on BST2. And we studiedthe expression of BST-2on peripheral blood mononuclear cells (PBMCs) from HIV-infected patients during the acute phases, chronic phases of disease as well as afterantiretroviral treatment (ART), and compared with uninfect PBMCs. The resultshowed that, expression of BST-2was decreased on mononuclear leukocytes HIV-infected patients than uninfected, and there has no obvious difference between theacute phases and chronic phases. |
PDF Full Text Request