Effect Of Disintergrin Kistrin On MMP-2 Protein Expression In Rabbit Lens Epithelial Cells After Extracapsular Lens Extraction

Objective In previous studies，we have already confirmed thatdisintergrin kistrin can inhibit the proliferation，adhesion and migration ofthe human lens epithelial cells (LECs) ,as well as decrease the expression ofcollagen IV in extracellular matrix(ECM) and the gene expression ofmatrix metalloprotein-2(MMP-2).So this study is to investigate the proteinexpression of MMP-2 by disintergrin Kistrin in lens epithelium cells(LECs)of rabbits after extracapsular lens extraction (ECLE) , and study themolecular mechanism of Kistrin.Methods All of the right eyes of 40 New Zealand adult rabbitsunderwent extracapsular lens extraction (ECLE) and the other eyes werenormal, then randomly divided into control group and experimentalgroup.0.2ml 80ng/ml Kistrin was injected into the capsular bag of lens inexperimental group while 0.2ml normal saline was into control group whenthe operation was over. After 1 day, 2 days, 3 days, 7 days, 14 days, 1month , 2 months and 3 months after the surgery ,the eyes were observedunder a slit lamp microscope to detect the Odrich classification of PCOgrading of the posterior capsule opacification. 10 rabbits of experimentalgroup and 10 of control group were executed on 14 days after the operation,marked as team A and team B. The others were killed on 3 months afteroperation, the experimental group played the role as team C, while thecontrol group as team D.10 left eyes were randomly chosen as blank controlgroup .Western blotting was used to detect the expressions of MMP-2 in allcases of capsule membranes . Western blotting relative gray value wereprocessed by Statistical software SPSS for Windows 13.0 image.Results Postoperative anterior observation: group A and B could notbe considered different（u=1.45,P=0.14）; group C and group D weresignificantly not the same; group C was lighter compared with group D( u=3.85,P=0.00）. Western blotting analysis results : MMP-2 relative gray value could not be detected in normal rabbit LECs. In the team A , theabundance was (0.378±0.019) compared to (0.769±0.011) in the team B，which decreased obviously（P=0.000) .The same thing happened in team Cand D，in team C was (0.361±0.013) while team D was (1.015±0.020)（P=0.000). Different stages after the operation showed a inflammatoryremarkable in control group（P=0.000），though no change in the expressionof MMP- 2 in experimental one (P=0.217).Conclusion Kistrin may play an important role in the expression ofMMP-2 after ECLE，which may be related to the decrease of collagen IVsecretion , as well as the profile of LECs and the collagen-secretion of ECM.