Expression And Significance Of β-defensin-2,-3and Interleukin-1β In Lesions Of Patients With Psoriasis Vulgaris

Objective To explore the expressions of β-defensin-2(HBD-2), β-defensin-3(HBD-3) and interleukin-1β (IL-1β) in the lesions skin tissues of psoriasis vulgaris and the influence of its common action on forming of psoriasis lesions.Methods By using real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT-PCR method) in15normal skin control group and45patients with psoriasis lesions, non-lesional skin tissue HBD-2、HBD-3and IL-1β expression.Comparison between different groups of HBD-2、HBD-3and IL-1β expression differences and correlation analysis.45patients with psoriasis lesions according to PASI score will be divided into mild group (25cases), moderate group (12cases) and severe group (8cases). Detection the HBD-2、HBD-3and IL-1β expression of mild group, moderate group and severe group lesions.Results The gene expression of HBD-2、HBD-3and IL-1β of the lesions skin tissues significantly increased than patients with psoriasis of the non-lesion tissues and the normal skin control group (P<0.01). The gene expression of HBD-2、HBD-3and IL-1β of non-lesion tissues in patients with psoriasis significantly increased than that of the normal skin in healthy people (P <0.01). The gene expression of HBD-2、HBD-3and IL-1β of the psoriatic lesion area in the mild group、the moderate group and the severe group were in turn increase, the comparison between two groups were statistically significant (P<0.01). In the lesions skin tissues of patients with psoriasis, the expression level of HBD-2、HBD-3、IL-1β and PASI score was a positive correlation (P<0.05).Conclusion The results suggest that HBD-2、HBD-3and IL-1β expression were increased in skin lesions of psoriasis vulgaris. and there were positive correlations between HBD-2and IL-1β、HBD-3and IL-1β, we assumed that HBD-2、HBD-3and IL-1β may be mutual interaction involved in the mechanism of psoriasis vulgaris to participate in the process.