The Detection Of T-Iymphocyte Subsets And Its Application In Patients Of Takayasus Arteritis、Rheumatoid Arthritis And Malignant Tumour

Objective:To investigate the change of T-lymphocyte subsets in patients of Takayasusarteritis, Rheumatoid arthritis and malignant tumour. and analyze their effects and significance in the diseases, further to investigate the pathogenetic mechanisms of the diseases.Methods:1.31patients with Takayasus arteritis as observation group were recruited,9men and22women;17-70years old, average47.45. The course of disease is2months-5years. All these accord with diagnostic criteria of brachiocephalic artery-type Takayasusarteritis. And30healthy persons as control group,14men and16women;20-64years old, average38.5. T-lymphocyte subsets (CD3+、CD4+、 CD8+) were detected by fluorescence microscope and humoral immunity(IgG、 IgA、IgM、IgE、C3、C4) were detected by BECKMAN IMMAGE800System. We will investigate the change of T-lymphocyte subsets in patients of Takayasusarteritis2.33patients with Rheumatoid arthritis as observation group were recruited,14men and19women;29-61years old, average43.6. The course of disease is3months-1year. and30healthy persons as control group,14men and16women;20-60years old, average38.5. T-lymphocyte subsets (CD3+、CD4、CD8+) were detected by fluorescence microscope. We will investigate the change of T-lymphocyte subsets in patients with Rheumatoid arthritis.3.38patients with malignant tumour as observation group were recruited,28men and10women;36-79years old, average57. and30healthy persons as control group14men and16women;20-64years old, average38.5. T-lymphocyte subsets (CD3+、CD4+、CD8+) were detected by fluorescence microscope. We will investigate the change of T-lymphocyte subsets in patients with. malignant tumour.Result1. The level of IgG、IgM、C3is1608.35±418.61,221.63±65.31,171.31±61.92(mg/dL) individually, the level of IgE is105.25±31.72(IU/ml), compared with healthy control1280.80±182.28,109.7±44.60, 104.78±17.73(mg/dL),45.61±40.38(IU/ml), the level of IgG、IgM、IgE、 C3、CD4+CD4+/CD8+in patients of Takayasus arteritis were increased significantly; the level of C4、CD3+in patients were decreased significantly.and there was no significant difference between the two groups about IgA and CD82. The level of CD3+、CD4+and CD4+/CD8+is63.00±4.48,32.25±3.76and1.08±0.13(%) individually, compared with healthy control,71.42±2.96,41.52±2.76,1.39±0.14(%), the level of CD3+、CD4+、CD4+/CD8+in patients of Rheumatoid arthritis were decreased significantly, and there was no significant difference between the two groups about CD8+3. The level of CD3+、CD4+and CD4+/CD8+is63.00±4.48,32.25±3.76and1.08±0.13(%) individually. Compared with healthy control,71.42±2.96,41.52±2.76,1.39±0.14(%), the level of CD3+、CD4+、CD4+/CD8+in patients of malignant tumour were decreased significantly, and there was no significant difference between the two groups about CD8+Conclusions1In patients with Takayasu arteritis, the function of humoral immune is in hyperthyroidism and the function of Cellular immune is in defect. In general, there is autoimmune disorders in patients with Takayasu arteritis.2In patients with Rheumatoid arthritis, the level of CD3+、CD4+、CD4+/CD8+were decreased significantly, it Indicated that the Lymphocyte proliferation and cytotoxic reactivity were declined.3In patients with malignant tumour, the level of CD3+、CD4+CD4+/CD8+were decreased significantly, it Indicated that the Lymphocyte proliferation and cytotoxic reactivity were declined.