The Clinical Study Between Syndrome Type In TCM And The Serum Levels Of IL-25and Total Ige In Patients With Bronchial Asthma

Objective:Bronchial asthma is one of the common and frequently occurring diseases in respiratory system. In traditional Chinese medicine (TCM), the types of bronchial asthma are diagnosed via four examination techniques including inspection, auscultation-olfaction, inquiry, and palpation. Therefore, the aim of the current research is to study the correlation between serum interleukin25(IL-25), total immunoglobulin E (IgE) and common clinical bronchial asthma with cold syndrome, bronchial asthma with heat syndrome, and deficiency syndrome of both lung and kidney, explore the material basis of TCM diagnosis of the syndromes, and provide scientific evidences for the diagnosis of the syndromes of TCM.Methods:60patients with bronchial asthma and20healthy volunteers were involved in the current study. The patients were devided into3groups according TCM theory, cold syndrome, heat syndrome, and deficiency of both lung and kidney groups, each group consisits of20subjects. Blood was collected from each subject and serum (2ml) was separated and stored at-80℃until IL-25and total IgE were measured. The IL-25level was detected by enzyme-linked immunosorbent assay (ELISA) method and the total IgE was determined by Chemiluminescence Method.Results:1. The level of serum IL-25of bronchial asthmatic groups were significantly higher than that of healthy control group (32.54±9.12, P<0.01). Although the level of serum IL-25in bronchial asthma with cold syndrome type was not statistically significant compared with the heat syndrome type (P>0.05), the IL-25level in bronchial asthma with cold syndrome type (59.16±12.94) and heat syndrome type (63.74±13.74) were significantly higher than those in the deficiency syndrome type of both lung and kidney (44.09±8.67, P<0.01).2. The level of serum total IgE of bronchial asthmatic groups were significantly higher than that of healthy control group (38.31±26.71, P<0.01). The level of serum total IgE in bronchial asthma with heat syndrome type (439.12±279.44) was significantly higher than that in the cold syndrome type (256.34±100.20, P<0.01). The total IgE level in cold syndrome type was markedly higher than that in the deficiency syndrome type (122.53±44.83, P<0.01). The level of serum total IgE in bronchial asthma with heat syndrome type was significantly higher than that in the deficiency syndrome type of both lung and kidney (P<0.01).3. In asthmatic groups, serum level of total IgE was positively correlated with that of IL-25(r=0.354, P<0.006)Conclusion:Some correlation was observed between serum IL-25and total IgE levels and bronchial asthma with TCM diagnosis of the syndromes. Serum IL-25, total IgE levels could be used as a reference for the diagnosis of TCM types of the asthma..