The Research On The Neurological Deficit Of Cerebral Ischemia In Rats After Rehabilitation Training Based On The Factorial Design

Objective:To provide a reasonable rehabilitation program by observing different time windows and different training intensity of rehabilitation training on the impact of neurological impairment after cerebral ischemia in ratsMethods:240rats were randomly divided into six groups, sham operation group, model group, the rehabilitation group A, the rehabilitation group B, the rehabilitation group C, the rehabilitation group D, according to3d,7d,14d,21d time point and then divided into four subgroup (n=10).Create a pMCAO model.Develop a rehabilitation training program based on the time window, the training intensity (2X2)factorial design, including the rehabilitation group C positive control group.At each time point,Balance beam test was used to evaluate the motor function,and TTC staining was used to detect the infarct volume,and immunohistochemical methods was used to detect the expression of NR2B protein in the cortex of ischemic area,and TUNEL method was used to observe the apoptosis of in the cortex of ischemic area.Results:1.Histopathology observation:Compared with sham,brain tissues loose, have significant vacuolar changes and deeply stained nuclear pyknosis,and the nucleolus was disappeared after pMACO. After rehabilitation treatment, brain tissue pathological have changes of varying degree in each rat.The cerebral edema and infarct area surrounding inflammatory infiltration in rehabilitation group D was lighter than other groups on7days14days and21days.2.Behavior observation:Compared with model group, the balance beam test scoresof each Rehabilitation groups were lower after surgery3days,and there was no statistically significant difference between the groups(p>0.05);On7days,14days,21days,the balance beam test scores of rehabilitation groups from low to high order:rehabilitation group D<rehabilitation group C(positive control)<rehabilitation group A <rehabilitation group B),and the balance beamtest score of rehabilitation of group C(positive control) and rehabilitation group D were better than the model group(p<0.05);On7days,14days, compared with the rehabilitation group C(positive control),the rehabilitation group D recovery of motor function was obvious,and there was statistically significant difference (p<0.05).3.The volume of cerebral infarction testing:The sham groups has no infarction.The infarct volume was reduced after rehabilitation training. Compared with model group,the volume of cerebral infarction of rehabilitation group C(positive control)and rehabilitation group D was significantly reduced at each time point,and the difference was statistically significant (p<0.05).Compared with rehabilitation group C (positive control), the volume of cerebral infarction of rehabilitation group D was significantly reduced on7days,14days,21days,and the difference was statistically significant (p<0.05).4.The expression of NR2B Protein:After rehabilitation training, the expression of NR2Bin each group was lower than the model group.On14days,21days,optical density values of NR2in ischemic cortex from low to high order in each group:the rehabilitation group D<rehabilitation group C (positive control)<rehabilitation group A<rehabilitation group<model group.On7days,14days,compared with rehabilitation group C(positive control),the expression of NR2B optical density values in ischemic cortex of rehabilitation group D was significantly reduced,and the difference was statistically significant(p<0.05). 5.Detection of apoptosis:there are a few TUNEL-positive cells in the sham group.At each time point.Compared with model group, TUNEL positive cells in each rehabilitation group were low.TUNEL positive cells in each group from low to high order:the rehabilitation group D<Rehabilitation group C(positive control group)<Rehabilitation group A<Rehabilitation group B<Model group.On7days,14days,21days,compared with rehabilitation group C(positive control),TUNEL positive cells in rehabilitation group Dwere significantly reduced,and the difference was statistically significant (p<0.05).Conclusions:1.Rehabilitation training can improve the motor function,reduce the volume of cerebral infarction after cerebral ischemia in rats.2.Rehabilitation training can reduce brain damage,which may reduce the apoptosis in ischemic areas by reducing NR2B expression after cerebral ischemia.3.The time window and intensity of rehabilitation training affect the outcome of rehabilitation.There is a risk in rehabilitation training after ischemia12hours.The high-intensity rehabilitation training can significantly reduce brain damage after ischemia,which was started from ischemia24hours.