| ObjectiveTo study the clinicopathological features of Clear cell renal cell carcinoma (CCRCC) and Chromophobe renal cell carcinoma (CRCC), to analysis the expression characteristics and the diagnostic value of CK7.Vimentin.Ki-67and CD34antibodies in two sets of data and discuss the significance of the various markers in the differential diagnosis.MethodsWe summarize and analyze68patients with CCRCC and30patients with CRCC who were treated at the Second Hospital of Tianjin Medical University. Tianjin Institute of Urology from2006to2010retrospectively on clinical and pathological characteristic. Immunohistochemistry (S-P method) was used to detect the expression characteristics of CK7, Vimentin, Ki-67and CD34in two sets of data. The results used for statistical analysis to determine whether there is statistically significant.Results1. CCRCC may show clinical and histological overlap with CRCC. It is not difficult to diagnosis conventional CCRCC and typical CRCC, but granular cell type or transparent and granular cell hybrid of CCRCC and eosinophilic variant of CRCC morphological easily be confused morphologically with each other.2. The expression of CK7:Collecting duct in the normal kidney tissue is locally positive. The positive rate is4.41%(3/68) in CCRCC tissue and100%(30/30) in CRCC tissue (P<0.01). The expression of CK7in CRCC tissue was no correlation with tumor stage and negatively correlated with histological grade (P<0.05).3. The expression of Vimentin:Proximal tubular in the normal kidney tissue is locally positive. The positive rate is100%(68/68) in CCRCC tissue and0%(0/30) in CRCC tissue (P<0.01). The expression of Vimentin in CCRCC tissue was positively correlated with tumor stage and histological grade (P<0.01).4. The expression of Ki-67:Positive staining was found in0%(0/40) of the normal kidney tissue. The positive rate is100%(68/68) in CCRCC tissue and76.67%(23/30) in CRCC tissue (p<0.01). The results of rank-sum test are that the proportion of positive and strong positive number of cases in CCRCC tissue was significantly higher than the CRCC (P<0.01).5.The amount of MVD in normal kidney tissue, CCRCC and CRCC tissue was respectively36.47±2.28,56.20±9.36,24.80±6.09, showing significant difference (P<0.05). In addition, tumor cell of CCRCC was separated by minute vessels and thick-walled blood vessels were visible in CRCC. Based on morphology, CCRCC may be distinguished from CRCC by CD34.6. The expression of Ki-67and MVD in CCRCC and CRCC was positively correlated with histological grade (P<0.01).Conclusions1. CCRCC and CRCC demonstrate different clinical behaviors, the correct diagnosis have obvious guiding significance to the clinical treatment. Experiments show that there was high expression of CK7in CRCC but only three cases in CCRCC. Vimentin was not stained in CRCC and stained in all CCRCC. CK7and Vimentin staining could serve as a useful adjunct in the differential diagnosis.2. Although expression of Ki67was found in both CCRCC and CRCC, the difference of positive expression intensity was statistically significant. Detection of Ki67may provide the basis for the differentia] diagnosis and contribute to judge the prognosis of disease.3. The difference of the amount of MVD between CCRCC and CRCC was statistically significant. And based on morphology, CCRCC may be distinguished from CRCC by CD34. Detection of Ki-67may contribute to judge the prognosis of disease.4. Through this experiment we learn more about CCRCC and CRCC in clinicopathology, the combination of CK7, Vimentin, Ki-67and CD34, especially in difficult cases, could serve as a useful adjunct in the differential diagnosis of CCRCC and CRCC. and provide some reference value for tumor occurrence, development and prediction of prognosis. |
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