Bear Bile Powder On Experimental Study Of Inhibition Of Liver Cancer

Aim:Through the experimental observation the effects of Bear bile powder to human liver SMMC7721cell proliferation, proved the effects of Bear bile powder on the H22inhibition of liver cancer in mice, and screened antitumor rate a higher dose. Clear the drug of liver cancer cell growth inhibition, preliminary discussion of the function of liver cancer Bear bile powder mechanism, to provide a theoretical basis for new drug development.Methods:Through the MTT method for the determination of human liver SMMC7721Bear bile powder cell proliferation influence; Establish mice portability tumor cells H22model, the mice by weight randomly divided into seven groups:blank array, positive control array, Bear bile powder100,200,400,800and1600mg/kg5dosage group, the daily fill the stomach to medicine,12consecutive days.24hours after discontinuation of cervical dislocated tumor-burdened mice to be put to death, stripping tumor tissue, were taken tumor, the thymus, spleen, liver weighing, calculation antitumor rate; Further screening, adjust the Bear bile powder dose, divided into200,400,500,600, and800mg/kg5dosage group, blank group, the positive control group unchanged, repeat three batch, antitumor rate of calculating the average.Results:To human liver SMMC7721Bear bile powder cell proliferation inhibition in24hours to reach a peak,24h Bear bile powder IC50=19.04ug/ml; Oral give Bear bile powder100(mg/kg·d.),200(mg/kg·d.),400(mg/kg·d.),800(mg/kg·d.),1600(mg/kg·d.) of liver cancer H22have inhibition, antitumor rate were36.00%,52.81%,61.44%,51.39%,45.96%. Further to Bear bile powder of liver cancer efficacy of three powder test, Bear bile powder200,400,500,600, and800mg/kg of liver cancer H22dose group of the average antitumor rate were47.13%,61.28%,51.82%,56.85%,60.56%.Conclusion:To human liver SMMC7721Bear bile powder cell proliferation in the influence of concentration dependence, inhibition reached a peak in24hours.100-1600mg/kg Bear bile powder five dose group of mice H22liver cancer of the antitumor rate were36.00%～61.44%between, of which400mg/kg,800mg/kg of liver cancer H22dose group of the average antitumor rate were61.28%,60.56%, and a good anti-cancer activity. In addition, the powder of liver cancer in mice H22Bear bile powder of weight, the thymus and spleen index, the index of liver index were not significantly influenced.