Stilbene Bei Recovery Drink For Jiajian Rat Myocardial Cell Apoptosis And The Effect Of Fas Fasl And Caspase 3 Protein Expression

Purpose：To observe the effect of Qibei Fuyuan Yin on myocardial apoptosisand expression of Fas, FasL and Caspase-3in Rats of Hypothyroidism.Material and method：The Rat of Hypothyroidism models were prepared byKolaja method.Healthy wistar Rats were randomly divided into Normal controlgroup,model group, Qibei Fuyuan Yin group, L-T4group. The morphological andultrastructural changes were observed under optical microscope and electronicmicroscope. Apoptotic cells were measured by TUNEL method.The expression ofapoptosis-related proteins Fas, FasL and Caspase-3were detected with immuno-histochemical methods.Results：1. Compared with control group，the apoptosis index and the expression of Fas,FasL and Caspase-3were reached the highest level in the model group2. Qibei Fuyuan Yin group and L-T4group were decreased significantly aftertreatment，and the effectivene ss of Qibei Fuyuan Yin group was better（p<0.05）.Conclusion：1. Hypothyroidism causes increase of myocardial cell apoptosis，heightenedexpression of Fas FasL and Caspase-3.2. Qibei Fuyuan Yin might decrease the expression of proteins Fas, FasL andCaspase-3，and inhibit the myocardial apoptosis.