The Associations Of L162V、C2528G、V227A Polymorphism Of The PPAR Alpha Gene With Early-onset Type2Diabetes Mellitus And Atherosclerosis

Objective：To explore the correlation of PPARαL162V、C2528G、V227Agene polyphism to early-onset T2DM and Atherosclerosis.Methods: A total of800（418men and382women）T2DM subjects wererecruited and divided into two groups：early-onset and late-onset groups which weredefined as diagnosed at≤40and≥40years of age, respectively. Early-onset T2DMsubjects400（211men and189women）. Late-onset T2DM subjects400（207menand193women）. Extracted peripheral blood white blood cell genomic DNA to detectPPARαL162V、C2528G、V227A polymorphism by the application of polymerasechain reaction（PCR）and calculate gene type and allele frequency of each group aswell as clinical biochemical indicators、incidence of atherosclerosis.Results：1.The fasting plasma glucose（FPG）, triglyceride（TG）, total cholesterol（TC）are higher and insulin resistance、inflammatory response more severe in early-onsetT2DM group than in late-onset T2DM grop.2.The overall frequencies of LL, LV, VV in early-onset T2DM group and inlate-onset T2DM group were91.5％,0％,8.5％, and97％,0％,3％respectively.The L162allele frequency is91.5％, and V162allele frequency is8.5％inearly-onset T2DM group, the L162allele frequency is97％, and V162allelefrequency is3％in late-onset T2DM group. There was statistical difference in thegene type frequency distribution between the two groups by check-up.3.The overall frequencies of GG, GC, CC in early-onset T2DM group and inlate-onset. T2DM group were93.5％,4.5％,2％, and97.2％,2％,0.8％respectively. The G2528allele frequency is95.8％, and C2528allele frequency is4.2 ％in early-onset T2DM group, the G2528allele frequency is98.3％, and C2528allele frequency is1.7％in late-onset T2DM group. There was statistical differencein the gene type frequency distribution between the two groups by check-up.4.The overall frequencies of VV, VA, AA in early-onset T2DM group and inlate-onset T2DM group were91.5％,8.5％,0％, and95％,5％,0％respectively.The V227allele frequency is95.7％, and A227allele frequency is4.3％inearly-onset T2DM group, the V227allele frequency is97.5％, and A227allelefrequency is2.5％in late-onset T2DM group. There was statistical difference in thegene type frequency distribution between the two groups by check-up.5.The early-onset T2DM group V162allele has higher FPG,2hPG, HbA1C, TC,TG, hs-CRP, HOMA-IR than the late-onset T2DM group L162allele. The early-onsetT2DM group C2528allele has higher TG than the late-onset T2DM group G2528allele. The early-onset T2DM group A227allele has higher HOMA-IR than thelate-onset T2DM group V227allele.6.The early-onset T2DM group V162allele has higher incidence ofatherosclerosis than the early-onset T2DM group L162allele、 the late-onset T2DMgroup L162allele and V162allele. The early-onset T2DM group C2528allele hashigher incidence of atherosclerosis than the early-onset T2DM group G2528allele、the late-onset T2DM group G2528allele and C2528allele. The early-onset T2DMgroup A227allele has higher incidence of atherosclerosis than the early-onset T2DMgroup V227allele、the late-onset T2DM group V227allele and A227allele. Therewas statistical difference in incidence of atherosclerosis between the early-onsetT2DM group V162allele、C2528allele、A227allele and other alleles by check-up.Conclusion：1.The early-onset T2DM group V162allele has poor blood glucose control, moredyslipidemia, more severe insulin resistance and inflammatory response than thelate-onset T2DM group L162allele.2. In the early-onset T2DM group PPARαL162V、C2528G、V227A allelesfrequencies are more elevatory than the late-onset T2DM group.3.The early-onset T2DM group V162allele has poor blood glucose control,more dyslipidemia, more severe insulin resistance and inflammatory response than thelate-onset T2DM group L162allele. The early-onset T2DM group C2528allele hasmore dyslipidemia than the late-onset T2DM group G2528allele. The early-onsetT2DM group V227allele has more severe insulin resistance than the late-onset T2DM group A227allele.4. PPARαL162V、C2528G、V227A polyphism may have associations to theearly-onset T2DM and Atherosclerosis.