| Objective：The purpose of this study is to explore the correlation of PPARapolymorphism to diabetic peripheral neuropathy in type2diabetes mellitus and riskfactors for diabetic peripheral neuropathy.Method：A total of1184T2DM subjects were enrolled and divided into DPNgroup(726cases) and NDPN group(458cases). Collected blood samples and extractedperipheral blood white blood cell genomic DNA to detect PPARaC2528G,PPARaL162V and PPARaV227A polymorphism by the application of polymerasechain reaction-restriction fragment length polymorphism(PCR-PFLP), and calculatedgenotype and allele frequency of each groups as well as differences. Compared theclinical indicators between DPN group and NDPN group as well as the clinicalindicators between different genotypes in each group.Result:1. BMI,2h-PG, glycosylated hemoglobin, triglyceride (TG) in the DPNgroup were higher than in NDPN group. The mean age of the patients in DPN groupwas higher and the patients in DPN group had a longer diabetes duration. Logisticregression analysis showed that diabetes duration, BMI, HBA1c and the increasedtriglyceride level were independent risk factors for diabetic peripheral neuropathy.2.The overall frequencies of CC, GC, GG of PPARaC2528G in DPN group and in NDPNgroup were2.5%,6.9%,90.6%and0.7%，5.5%，93.8%respectively. The C2528allelefrequency was5.9%，and G2528allele frequency was94.1%in DPN group; the C2528allele frequency was3.4%, and G2528allele frequency was96.6%in NDPN group.There was statistical difference in the genotype frequency distribution between the twogroups by check-up.3. The overall frequencies of VV, LV, LL of PPARaL162V inDPN group and in NDPN group were6.3%，0%，93.7%and3.3%,0%,96.7%respectively. The V162allele frequency was6.3%, and L162allele frequency was 93.7%in DPN group; the V162allele frequency was3.3%, and L162allele frequencywas96.7%in NDPN group. There was statistical difference in the genotype frequencydistribution between the two groups by check-up.4. The overall frequencies of VV, VA,AA of PPARaV227A in DPN group and in NDPN group were90.2%,9.8%,0%, and86.2%,13.8%,0%respectively. The V227allele frequency was95.1%, and227A allelefrequency was4.9%in DPN group; the V227allele frequency was93.1%, and227Aallele frequency was6.9%in NDPN group. There was statistical difference in thegenotype frequency distribution between the two groups by check-up.5. In DPN group,the patients who carried the C2528allele had higher serum concentrations oftriglyceride, total cholesterol, low density lipoprotein cholesterol and apolipoprotein Bthan the ones who carried the G2528allele; In NDPN group, the patients who carriedthe C2528allele had higher serum concentrations of triglyceride, total cholesterol, lowdensity lipoprotein cholesterol, fasting plasma glucose, postprandial blood glucose, andhigher glycosylated hemoglobin than the ones who carried the G2528allele.6. In DPNgroup, the patients who carried the V162allele had higher serum concentrations of totalcholesterol, low density lipoprotein cholesterol, apolipoprotein B, fasting plasmaglucose, and higher glycosylated hemoglobin than the ones who carried the L162allele;in NDPN group, the patients who carried the V162allele had higher serumconcentrations of total cholesterol, triglyceride, fasting plasma glucose than the oneswho carried the L162allele.7. In DPN group, the patients who carried the227A allelehad lower serum concentrations of triglyceride and total cholesterol than the ones whocarried the V227allele; in NDPN group, the patients who carried the227A allele hadlower serum concentration of total cholesterol than the ones who carried the V227allele.Conclusion: Age, diabetes duration, BMI, postprandial blood glucose, HBA1c,triglyceride were related to diabetic peripheral neuropathy; diabetes duration, BMI,HBA1c and the increased triglyceride level were independent risk factors for diabeticperipheral neuropathy. There was statistically significant correlation betweenPPARaC2528G polymorphism and diabetic peripheral neuropathy; PPARaC2528Gpolymorphism was associated with the serum lipid concentrations of diabetic peripheralneuropathy; C2528variation was possibly a risk factor for diabetic peripheralneuropathy. There was statistically significant correlation between PPARaL162Vpolymorphism and diabetic peripheral neuropathy; there was a significant association ofPPARaL162V polymorphism with serum lipid concentrations of diabetic peripheral neuropathy; V162variation was possibly a risk factor for diabetic peripheral neuropathy.There was statistically significant correlation between PPARaV227A polymorphismand diabetic peripheral neuropathy; PPARaV227A polymorphism was associated withthe serum lipid concentrations in DPN group;227A variation was possibly a protectivefactor for diabetic peripheral neuropathy. |
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