The Relationship Between Adipose Tissue TLR4/NF-κB Signaling Pathway And High-fat Diet Induced Insulin Resistance In Mice

Objective:Insulin resistance in obesity is associated with a chronic systemiclow-grade inflammation. The study aims to explore the relationship between theTLR4/NF-κB signaling pathway and high-fat diet induced insulin resistance in miceadipose tissues. Methods:C57BL/6mice (80) were randomly divided into two groups,which were feeded with normal diet and high fat diet respectively, and then randomlydivided into eight groups according to the feeding days(0,3,7,11,14,21,42,84daysrespectively).The weight, fasting blood glucose and serum insulin levels were detected,and the adipose tissue was observed through tissue slice with HE.Immunohistochemistrywas used to detect the expression of inflammation factors such as TNF-α, IL-6so as todescribe the formation and development of fat local inflammation. Finally, theWestern-blot method was adopted to detect proteins of TLR4/NF-κB.Results:The resultsshowed that the mice insulin resistance model were successfully created with high-fat diet,and high-fat diet induced the expression of TLR4mRNA and protein in adipose tissue.TLR4/NF-κB signaling pathway activation increased gradually with days of high-fat dietgiven,and which reached a maximum level at the fifth day.The adipose tissue increasedTNF-α and IL-6expression in parallel,and the fat cells became bigger.Conclusion:Thehigh expression of TLR4/NF-κB in adipose tissue and the increasing of serum insulin isconsistent.The high-fat diet can activated fat cells TLR4/NF-κB signaling pathways,andhave the association with the insulin resistance.