Study On The Dendritic Cell Subsets Function And Its Relationship With The Expressions Of Gata-3and T-bet In Lymphocytes In Immune Thrombocytopenia

Objective To investigate the relationship between the dendritic cell (DC) subsets and abnormal expression of transcriptive factors, Gata-3and T-bet of the patients with immune thrombocytopenia.Methods1.The expression of CD80and CD86on myeloid DC (mDC, HLA-DR+Lin-CD11c+cells) and plasmacytoid DC (pDC, HLA-DR+Lin-CD123+) of54ITP (22untreated and20remitted) patiens and12healthy controls were analyzed by flow cytometry.2.The plasmacytoid DC (pDC) and myeloid DC (mDC) of45ITP (16untreated and17remitted) patients and12healthy controls were analyzed by flow cytometry (FCM). The expressions of Gata-3mRNA and T-bet mRNA in PBMNC were detected by RT-PCR.The level of IL-4and IFN-γ were measured by FCM in33ITP patients and12healthy controls.Results1. The percentages of mDC in PBMNC were (0.22±0.20)%and(0.29±0.16)%in untreated and remitted ITP patients respectively no statistic difference in healthy controls.(0.30±0.11,p>0.05). The percentages of pDC in PBMNC were (0.59±0.11)%and (0.47±0.12%) in untreated and remitted ITP patients respectively,which were both higher than that in healthy controls (0.32±0.13%,P＜0.05). pDC/mDC ratios in untreated which was (3.18±1.83) and in remitted (2.36±2.08) were higher than that in healthy controls(1.13±0.46)(P＜0.05). No statistic difference in pDC/mDC ratio were found between in untreated and in remitted (P>0.05). The expression of CD80on pDC was (15.01±12.28)%in untreated ITP patients and higher than that in remitted (7.30±6.47)%and in healthy controls(2.53±2.36)%(P＜0.05). there was higher in remitted than that in healthy controls (p＜0.05). The expression of CD86on pDC was (75.48±18.48)%in untreated ITP patients in remitted(56.83±13.52)%and higher than that in healthy controls(47.33±19.76)(P<0.05). No statistic difference were found between in untreated and in remitted patients (P>0.05). The expression of CD80on mDC was (8.12±1.96)%in untreated and higher than that in remitted ITP patients (3.73±1.89)%and in healthy controls (2.46±1.34)%(P＜0.05). No statistic difference were found between in remitted and controls (P>0.05). The expression of CD86on mDC was (97.49±1.99)%in untreated ITP patients, in remitted (94.10±5.90)%and in healthy controls (92.49±11.08)%. No statistic difference were found among the three groups (P>0.05).2. The percentage of pDC in PBMNC was (0.49%±0.18%) in untreated,which was higher than that in remitted ITP patients (0.27%±0.17%) and in healthy controls (0.32%±0.13%)(P<0.05).The percentage of mDC in PBMNC was (0.49±0.18)%, in untreated was (0.29±0.16)%and (0.30±0.11)%in controls,There was no statistic difference of mDC expression among the3groups(P>0.05).pDC/mDC ratios was (3.15±2.07)%in untreated ITP patients, which was higher than that in remitted ITP patients (0.81±0.32)%and in controls (1.07±0.44)%(P＜0.05). The relative mRNA expression levels of transcriptive factor Gata-3of the three groups were2775±489,1357±307and652±165respectively. Gata-3mRNA expression in untreated group and in remission group was higher than that in healthy controls(P<0.05). The relative mRNA expression levels of transcriptive factor T-bet were782±394,583±176and576±120.There was no statistic difference of T-bet expression among the3groups (P>0.05). Gata-3mRNA/T-bet mRNA ratio was(4.13±1.69) in untreated group,which was higher than that of remission group (2.45±0.69) and healthy controls (1.15±0.27)(p<0.05). The level of IL-4in the untreated group was higher than that of in remission group and healthy controls [(9.14±4.34)%,(4.78±1.69)%,(4.86±1.41)%, p<0.05]. The level of IFN-Y in the three groups were [(0.97±0.75)%,(3.75±1.49)%、(3.58±1.33)%]. The level of IFN-γ in untreated group was lower than that in remitted ITP patients and healthy controls,p<0.05.There was significant positive correlations between Gata-3and pDC/mDC (r=0.585, P<0.01),and positive between Gata-3and IL-4(r=0.463P<0.05). No correlation between T-bet and pDC (p>0.05), so were T-bet and pDC/mDC(P>0.05), T-bet and IFN-γ (P>0.05).Conclusion The imbalance of DC subsets, increasing expression of costimulatory factor and the higher expression of downstream transcription factor Gata-3mRNA play an important role in the pathogenesis of ITP.