The Frequency And Function Of Blood And Salivary Gland CD4+CXCR5+Tfh Cells In The Patients With Primary Sjogren’s Syndrome

Objective To investigate the frequencies and the role of CD4⁺CXCR5⁺T cells in theCD4⁺T cells from peripheral blood and salivary gland of patients with primarySjogren’s syndrome （pSS）Methods Frequencies of blood CD4⁺CXCR5⁺T cell，CD19⁺CD27-na ve B cell、CD19⁺CD27⁺memory B cell and CD19⁺CD27^highplasmacell were analyzed by flowcytometry of24pSS patients before and after treatment and20healthy controls.Differences between groups and influences of treatment were analyzed. Meanwhile, theexpression of CD4⁺CXCR5⁺T cell，CD19⁺CD27-na ve B cell、CD19⁺CD27⁺memory Bcell and CD19⁺CD27^highplasmacell on salivary gland with patients of pSS withouttherapy was analyzed. Independent sample T test, person correlation analysis were usedfor statistical analysis.Resultsâ‘ The frequency of peripheral blood of CD4⁺CXCR5⁺in CD4⁺T cells andCD19⁺CD27-naive B cells in CD19⁺.B cells were increased in patients with pSS withouttreatment compared with healthy controls（17.90±4.40%vs.14.45±3.54%, P=0.019;（72.28±12.94%vs64.65±10.21%, P=0.043），）.The percentage of peripheral blood ofCD19⁺CD27⁺memory B cells and CD19⁺CD27^highplasma cells in CD19⁺B cells werereduced in patients with pSS without treatment compared with healthy controls（26.71 ±12.72%vs33.73±12.24%, P=0.059ï¼›1.08±0.58%vs1.61±0.62%, P=0.008）.â‘¡Thefrequency of peripheral blood of CD4⁺CXCR5⁺Tfh cells had no correlations with Thefrequency of peripheral blood of CD19⁺CD27⁺memory B cells and CD19⁺CD27^highplasma cells.â‘¢No correlations was found between clinical index such as ESR andpercentage of peripheral blood of CD4⁺CXCR5⁺T cells in patients with pSS withouttreatment（P>0.05）.â‘£The frequency of salivary CD4⁺CXCR5⁺Tfh cells havesignificant correlation with CD19⁺CD27⁺memory B cells and CD19⁺CD27^highplasmacells. And the percentages of salivary CD19⁺CD27^highplasma cells have positivecorrelation with serum ANA titers.⑤The percentages of peripheral blood ofCD4⁺CXCR5⁺T cells were decreased （（15.19±3.23）%）after being treated for4weekswith prednisone （≤10mg/d） compared with those before receiving treatment（（18.07±4.25）%）.Conclusions These results demonstrate that the abnormality of CD4⁺CXCR5⁺T cellsmay play an important role in the pathogenesis of pSS.