| Objective:Through observing the changes of gastrointestinal hormones and interstitial cells of cajal in the diabetic gastroparesis (DGP) experimental rats before and after the Banxia Xiexin Decoction treatment, the role played by the xinkaikujang method in rebuilding the basic unit of gastric motility mechanism of diabetic gastroparesis is tentatively presented.Method:120rats were used as experimental subjects, among which10rats were set aside as the blank group, and the remaining110are modeled by using STZ modeling method. After successful modeling,78of the successfully modeled DM rats are selected and are divided into6groups, namely, model group, domperidone group, rnetformin group, Banxia Xiexin Decoction high dose group, Banxia Xiexin Decoction middle dose group, Banxia Xiexin Decoction low dose group. The gavage starts on the second day. The blank group and the model group are given distilled water, and the water intake is observed. On the0th and7th days, weighing the rats, dissecting the rat antral quickly to take full thickness, measuring the rate of experimental rat gastric emptying, intestinal propulsion, VIP, SS, SP, MOT data are undertaken and the data are recorded and analyzed by statistics method. Meanwhile, the use of immunohistochemical techniques for c-kit positive ICC morphology was observed and counted.Results:①Model group of diabetic gastroparesis demonstrates delayed gastric emptying time and decreased intestinal propulsion rate, and the SS, VIP content expression is increased while SP, MOT are reduced compared with the control group (p<0.05). The results indicate, that increased SS, VIP content expression, SP, MOT reduction are the main mechanism causing diabetic gastroparesis.②Low, middle and high doses of Banxia Xiexin Decoction can promote the secretion of motilin, but with the increase of drug dose, motilin is lowered instead, showing an inverse relationship. The low-dose Banxia Xiexin Decoction group compared with the control group (p>0.05) indicates the low dose Banxia Xiexin Decoction enables the motilin level of diabetic gastroparesis rats to return to a healthy level.③After pairwise comparisons, no significant difference is detected in the intestinal propulsion rate of the control group and domperidone group, Banxia Xiexin Decoction low dose group, Banxia Xiexin Decoction high dose group (p>0.05). This result shows that domperidone, Banxia Xiexin Decoction low and high dose groups can only enhance gastric motility in diabetic gastroparesis rats while producing literally no effect on intestinal propulsion rates. The Banxia Xiexin Decoction middle dose group can improve intestinal propulsion rate, but compared with the metformin group (p<0.05), there are significant differences, indicating that middle dose is not as efficacious as the metformin group.④The VIP level of the model control group, when compared with the Banxia Xiexin Decoction low-dose group (p>0.05) shows no significant difference. The pairwise comparisons with domperidone group, metformin group, Banxia Xiexin Decoction middle dose group and high dose group results in p<0.05, but the pairwise comparisons among these latter four groups show no significant difference (p>0.05). This shows that low-dose Banxia Xiexin Decoction does not reduce VIP level, while middle and high doses of Banxia Xiexin Decoction can achieve the same effect as domperidone and hypoglycemic drugs even with a decrease VIP level.⑤The comparison between the SP substances of the Model group and domperidone group (p>0.05) demonstrates that domperidone is ineffective in improving the SP substances, but there is a significant difference when compared with low, medium and high doses Banxia Xiexin Decoction group and metformin group (p<0.01), indicating various doses of Banxia Xiexin Decoction and metformin increased SP substance is effective. Pairwise comparisons among metformin group and low, middle and high dose Banxia Xiexin Decoction show no significant differences (p>0.05), which suggest that the efficacy of the metformin group is similar to that of the low, middle and high doses of Banxia Xiexin Decoction.⑥he effect of high dose Banxia Xiexin Decoction in reducing SS was the best, and compared with domperidone group (p<0.05), there are significant differences, indicating that the efficacy of high-dose group is better than domperidone group. The comparison between the high-dose Banxia Xiexin Decoction group, metformin group, Banxia Xiexin Decoction low dose group, and middle dose Banxia Xiexin Decoction group (p>0.05) shows no significant difference, indicating that the middle and high doses of Banxia Xiexin Decoction do not contribute to the deduction of SS level.⑦C-kit immunohistochemistry:the findings show ICCs Immunohistochemical chromosomes are positive and brown granules of cytoplasm are visible. ICCs are mainly distributed in the digestive tract between the autonomous nerve endings and smooth muscle cells, spindle-shaped or stellate, large nuclei, oval, chromatin dispersion, little cytoplasm. The number of gastric antrum ICC of the diabetic gastroparesis model rats is obviously larger than the healthy rats. The number of gastric antrum ICC of the Banxia Xiexin Decoction treated rats is significantly increased.Conclusion:Banxia Xiexin Decoction treatment of diabetic gastroparesis rats, through effective regulation of VIP, SS, SP, MOT level, can also improve gastric emptying and intestinal propulsion. Meanwhile, after Banxia Xiexin Decoction rats treatment, the number of antral positive ICC is significantly increased. |
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