Study On The Reversion Resistance Of Ovarian Cancer By Curcumin

Objective:According to the study on the effects of curcumin on human ovarian cancerA2780/Taxol cells, the mechanism of cell resistance into the cell resistance andcurcumin reversed can be figured out. Finally, the reversal effect of curcumin resistantapplications provides an experimental basis for clinical.Methods:1、Ovarian cancer cell A2780/Taxol in vitro, and MTT colorimetry detects thatcurcumin with different concentrations influence on proliferation of MTT.2、Curcumin and paclitaxel effect on ovarian cancer cells A2780/Taxol, and dyeing byPI (propidium iodide), the flow cytometry instruments measure cell apoptosis.3、 Western-blot method detects the expression of P-gP, PKC-α after curcumineffecting on human ovarian cancer A2780/Taxol cells.Results:1、The combination of paclitaxel and curcumin with different concentrations effectovarian carcinoma cells A2780/Taxol on impeding the cell growth more obviouslycompared with single effect of paclitaxel （P<0.01）; when Curcumin concentration isless than20μ M, with increasing of the curcumin concentration, human ovarian cancerA2780/Taxol cells growth inhibition increased gradually, and the difference hasstatistical significance（P<0.01）; when Curcumin concentration is up to20μ M, theconcentration of curcumin on human ovarian cancer A2780/Taxol does not haverelationship of cells growth inhibition rate, and the differences does not have statisticalsignificance（P>0.05）. 2、The results of western blot show that Mdr-1/P-gP, PKC-protein expression is lowerthan that of the control group after human ovarian cancer A2780/Taxol cells interposedby curcumin, and with increasing of curcumin concentration, the Mdr-1/P-gP, PKC-protein expressions are decreased obviously; when curcumin concentration is increasedto20μ M, the Mdr-1/P-gP, PKC-protein expressions are not decreased any more.3、After PI staining of cells in different experimental groups, the detection results ofthe flow cytometry instruments have found that the apoptosis rates of curcumin groupcells are increased more obviously than the control group（P<0.05）; When curcuminconcentration is less than to20μ M, and with increasing of the curcumin concentration,human ovarian cancer A2780/Taxol cells apoptosis rates have a tendency to increase,and the difference has statistical significance（P<0.01）; when Curcumin concentrationis up to20μ M, the concentration of curcumin on human ovarian cancer A2780/Taxoldoes not have relationship of cells growth inhibition rate, and the differences does nothave statistical significance（P>0.05）; The apoptosis rate that using20μ M curcuminin combination with paclitaxel group is significantly higher than that using paclitaxelgroup alone, the difference has statistical significance（P<0.01）.Conclusions:1、Curcumin can inhibit human ovarian cancer A2780/Taxol cells growth and anti-tumor effect. The combination of paclitaxel and curcumin effect on impeding the cellgrowth more obviously compared with single effect of paclitaxel. Curcumin canincrease the cytotoxicity of ovarian carcinoma cells A2780/Taxol from paclitaxel, andreduce the cell resistance.2、Curcumin could inhibit Mdr-1gene expression in human ovarian cancerA2780/Taxol, reduce P-gP protein expression, impede biological pump function of P-gPprotein, reduce chemotherapeutic drugs in the pump from ovarian cancer cells, andincrease paclitaxel sensitivity from cells. 3、Curcumin could reduce PKC-protein expression in human ovarian cancerA2780/Taxol, decrease P-gp phosphorylation rate, inhibit the biological function of P-gP protein, and reverse ovarian cancer resistance mediated by P-gP.