Studies On The Chemical Constituents And Bioactivities From Flowers Of Ailanthus Altissima Swingle

Objective: To isolate and identify the major chemical constituents from the flowersof Ailanthus altissima (Mill.) Swingle. Besides, to do some primary research of theeffect on cytotoxicity of some kinds of constituents.Methods: Macroporous absorptive resins (DM130), Silica gel, Sephadex LH-20,ODS, HPLC were employed for the isolation and purification of chemicalconstituents. The structures were identified on the basis of spectral data andphysicochemical examination. Three kinds of constituents were chosen to do someprimary research of the effect on cytotoxicity and the effect on A549cell cycle.Result:18compounds were isolated and identified as follows: scopoletin (I),ursolic acid (II), palmitic acid (III),(+)-isolariciresinol (IV), daucosterol (V), ethylgallate (VI), β-Sitosterol (VII), Azelaic acid (VIII), brevifolin (IX), Rutin (X),diethyl-2,2’,3,3’,4,4’-hexahydroxybiphenyl-6,6’-dicarboxylate (XI), brevifolincarboxylic acid (XII), ellagic acid (XIII), methyl brevifolin carboxylate (XIV),Quercetin (XV), kaemferol (XVI), gallic acid (XVII),3,4,8,9,10-pentahydroxy-dibenzo-[b, d]pyran-6-one(XVIII). Compound XIX--compound XXIII are notidentified.The experimental results show that the three kinds of constituents have someeffect on cytotoxicity and A549cell cycle.Conclusion: Compounds brevifolin (IX), diethyl-2,2’,3,3’,4,4’-hexahydroxy-biphenyl-6,6’-dicarboxylate (XI), brevifolin carboxylic acid (XII), ellagic acid (XIII),methyl brevifolin carboxylate (XIV)were isolated from this genus for the first time. Compounds I～XXIII are isolated from the flowers of Ailanthus altissima (Mill.)Swingle for the first time. The three kinds of constituents have certain effect oncytotoxicity and A549cell cycle, which IC50are43.5μM,31.2μM,42.1μM.