Preparation And Combined Effects Of Cucurbitacin B And Furazolidone On Alcohol Dependence Animal

ObjectiveVomiting were observed after the administration of experimental animal caused by drinking;using high performance liquid chromatography method for the determination of content of ACE-DNPH after administration of experimental animal serum, analyze the relationship between acetaldehyde concentration in serum of experimental animal and vomiting;study on the preparation and drinking effect.Preparation of cucurbitacin B and furazolidone compound film coated tablets.MethodsThe experiment of alcohol dependence animal feeding, were divided into5groups(n=2).Respectively, cucurbitacin B group (positive control group), furazolidone group (positive control group), cucurbitacin B combined with furazolidone and middle dose, the dose of cucurbitacin B combined with furazolidone in and cucurbitacin B combined with furazolidone. Dose To the drug after a week, giving each experiment animal60degrees60degrees liquor liquor80ml, cat50ml, a drinking.To observe the service level and number of vomiting reaction within1.5h after drinking.The establishment of animal model of alcohol dependence, the drug caused by alcohol dependent animal drinking after the vomiting frequency and degree and alcohol effect);HPLC method for the determination of administered before and after drinking in30,60,90minites, drug animal serum of ACE-DNPH concentration in the animal experiment.L-2000Elite high performance liquid chromatography, chromatographic column:Hypersil ODS2C18, column temperature:35℃, mobile phase of methanol:water=80:20, the detection wavelength was360nm,the flow rate was1ml/minThe amount of melon stalk crushing research into coarse powder,10times the volume of water, extracted3times, each time1.5h, filtering, merging the extracted liquid, filtering, removing dregs, to obtain concentrated solution.To concentrate in slow and absolute alcohol, and stir, to make the alcohol content reached70%, static36h, to obtain supernatant, alcohol precipitation filtration, filter residue with equal volume of ethanol washing and filtering, merging filtrate. Recovery of alcohol, concentrating, vacuum drying, dry paste, as the prescription.Prescription two furazolidone tablets amount tablets are crushed, added prescription, mixed15mesh sieve.Add the mixture material (soluble starch:microcrystalline cellulose=1:1, the amount of2.11times the mixed extract),85%alcohol, magnesium stearate amount, sugar amount, whole grain, compression.Liquid acrylate resin with a spray gun to spray coating pan uniform No. IV, slow drying48h, after curing the prepared products.Study of cucurbitacin B and furazolidone compound film coated tablet preparation process, through the investigation of the use of different materials affect tablet forming process, the best prescription screening and optimizing the preparation.ResultsAfter90days after drinking, experimental animal has the effect of dependence on alcohol, for the performance of selective drinking water containing alcohol.Use of cucurbitacin B and furazolidone alcohol dependent animal drinking than simply taking the cucurbitacin B, animal vomiting furazolidone obviously (P<0.01). Application of high performance liquid chromatography method for the determination of ACE-DNPH in animal serum, its linear relationship with peak area in4-10μg/ml range (n=7,r=0.9993), the average recovery rate was0.98%.The ACE-DNPH furazolidone group animal drinking had higher serum, cucurbitacin B and furazolidone group animal low.The tablet core of cucurbitacin B and furazolidone to optimization of the prescription, to cucurbitacin B and furazolidone for the tablet core formulation, the main sugar, magnesium stearate as auxiliary materials, to IV of acrylic resin as coating material, film coating tablet preparation.In the process of preparation, constantly optimize the compound coating prescription, so the medicine can play the biggest emetic action.ConclusionsHigh performance liquid chromatography method for the determination of ACE-DNPH in animal serum of strong specificity, high sensitivity, good reproducibility;Cucurbitacin B and furazolidone combination on alcohol dependent animal drinking after vomiting has synergistic effect;Cucurbitacin B furazolidone unrelated to alcohol and acetaldehyde vomiting blood concentration dependent animal after drinking. Cucurbitacin B and furazolidone combined with emetic action is better than simple use of furazolidone or cucurbitacin B has effect on animal animal after drinking alcohol dependence, showed a dependence of therapeutic effect in patients with potential to alcohol, has a good application prospect.Cucurbitacin B and furazolidone compound film coated tablet preparation process is reasonable and feasible, product stability.