The Effect Of ChangQiang Acupuncture On The NLGN-3Protein Expression On Which VPA Autism Model Of Rat’s Hippocampus

Objective: to observe the acupuncture Changqiang on autism in the rat hippocampus NLGN-3protein expression.Methods:(1) clean level each10Wistar male and female rats. Establish the VPA autism model in rats.24the VPA model group, blank control group (group A)8. In the two groups were is weight determination and the direction of development of tendency of functional testing, behavior to harm perception test; Will be24in the VPA group were only through the random number table method is divided into model control group (group B) eight, eight strong long treatment group (group C), the acupuncture point treatment group (group D)8.(2) in group C and group D were the next day for acupuncture treatment after ablactation, select long hole, group C group D choose the hole. Acupuncture and depth is about0.3inches, the needle jump meddling in the method, frequency is about160-200times/min, for a minute. Acupuncture1times a day,10days for a period of treatment,2days off after a period of treatment and acupuncture, acupuncture three consecutive period of treatment. Normal control group (group A) with the model control group (group B) only grasp motion simulation acupuncture group, with the former. Intervention after the course of the next day, the experiment water maze test in rats, and the next day after the water maze test in the brain, brain tissue by immunohistochemistry technique to detect hippocampal NLGN grey value-3positive cells. The experimental data using the single factor analysis of variance and correlation analysis.Results:(1) children were in the determination of:(1) weight determination: PN7blank control group (14.21+/-1.85), the VPA model group PN7(13.98+1.83), and blank control group PN14(25.34+/-2.80), the VPA model group PN14(25.64+2.41), and blank control group PN21(37.4PN21(37.4+/-3.28), the VPA model group (36.62+3.61)PN7p=0.489>0.05; PN14p=0.895>0.05; PN21p=0.371>0.05there were no statistical significance;(2) the direction of the tendency of test: PN7blank control group (152.34+/-47.54), the VPA model group PN7(228.50+98.42); PN8blank control group (130.81+/-42.60), the VPA model group PN8(192.46+76.42); Blank control group PN9(78.16+/-38.25), the VPA model group PN9(139.07+79.61); PN10blank control group (55.04+/-31.57),the VPA model group PN10(108.68+94.72)、PN7p=0.001<0.00PN8p=0.0500<0.05; PN9p=0.000<0.05; PN10p=0.000<0.000all have significant differences,blank control group in the direction of time trends are superior to the VPA model group.(2)damage perception test:in PN9(p=0.010<0.010), PN11(p=0.003<0.05), PN13(p=0.05<0.05) and PN15(p=0.269>0.05), the VPA model pain threshold than the blank group, and in PN9, PN11and PN13have significant difference. There was no statistically significant difference in PN15two groups.(3) the water maze test:(1) average escape latency period respectively after acupuncture intervention group A (13.64+/-1.72) and group B (30.32+8.32),(14.56-3.05) of group C, D group (26.78+/-5.34).The average latent period of A and C group was obviously shorter than group B (P<0.05), there is statistical significance;(2) the water maze fifth day after the acupuncture intervention through the platform number respectively is:group A (2.25+/-0.46) and group B (0.87+0.64),(1.87-0.64) of group C, D group (0.87+/-0.35). A, group C cross platform significantly more than group B (P<0.05), with statistical significance.(2) the hippocampus NLGN-3protein positive cells grey value test results:group A (137.76+/-6.65) and group B (189.14+8.59),(175.05-6.71) of group C, D group (187.67+/-8.66). A significantly less than group C and group D (P<0.05).(3) acupuncture intervention after long strong group of experiment data correlation analysis:(1) the average escape latency and hippocampus NLGN-3positive cells grey value positive correlation (r=0.928,P<0.05);(2) through the platform number is negatively related to the grey value NLGN-3positive cells (r=0.838, P<0.05).