Effects Of Buyang Huanwu Decoction On Caspase-3of Post-stroke Depression In Rats

Objectives:To explore the effects of Buyanghuanwu Decoction on programmed cell death in post-stroke depression animal model rats.Methods:The mouse model of focal cerebral ischemia was established by means of middle cerebral artery occlusion. Then three weeks of salute-living and chronic mild unpredictable stress was given to the animal after cerebral stroke to induce the post-stoke depression animal model. The adult rats were randomly divided five groups, including the normal group, shame-operated group, post-stroke group, Buyanghuanwu decoction group and fluoxetine group. Animals were received different treatment. All the rats will be observed at7,14,21days point after operation. The traveled distance, speed-average (without resting time), sugar consumptions and neurological severity score and caspase-3of cerebral cortex and CA2were measured by immunohistochemical technology. And observe the effects of Buyanghuanwu decoction.Result: 1. When the model of post-stoke depression was established, the traveled distance, speed-average (without resting time), sugar consumptions were all decreased; but after treatment of Buyanghuanwu decoction, they were all increased.2. The neurological severity score of rats were increased after post-stoke depression, but treatment of Buyanghuanwu decoction, it is decreased3. Compared with the normal group and shame-operated group, the caspase-3of cerebral cortex and CA2were increased. But treatment of Buyanghuanwu decoction, it is decreased.Conclusion:1. Buyanghuanwu decoction can treat the post-stroke depression, and promote the PSD recovery of neurological function in rats.2. Buyanghuanwu decoction can inhibit the expression of caspase-3to reach the effect for the treatment of post-stroke depression.