Studies On Hypoxia Inducible Factor-1α And PC-cell-derived Growth Factor In Nasopharyngeal Carcinoma And Their Clinical Significances

BackgroundNasopharyngeal carcinoma, occurring in nasopharyngeal mucosa epithelial cancer, is one of the most hackneyed incidences of malignant neoplasm. According to the survey by World Health Organization in2010, the global average incidence of nasopharyngeal carcinoma (NPC) is about2.28/10among the men, women of1.12/10. Similar to previous statistics, China’s NPC patients still account for about80%of the world’s, with the sex ratio of2.06:1; same to other cancers, the age group is increasingly younger theses years.The clinical manifestation of Nasopharyngeal carcinoma is complicated with no specificity. The early symptoms are not typical, which means the misdiagnosis is of high possibility. It has two kinds of invasive ways:ascending and descending, both of which are powerful in affecting and transferring. Over60percent of patients have already exacerbated to the terminal condition, which is of high malignance, with the pathology of poorly differentiated squamous cell carcinoma and undifferentiated carcinoma.Hypoxia is the common characteristic of entity tumor cells. Under anoxic environment, many genes, stimulated by anoxia, procured the proliferation and shift of the cancer cells. HIF1-α is one of the key proteins control the reaction.A large number of research data and the experimental results show that:in many solid tumors, PCDGF can activate MAPK, ERK1/2and integrin to further promote the formation and invasion of various types of solid, and lowering the level of it can significantly inhibit the formation of tumor microvascular and progress of invasion.Detection of HIF-1α and PCDGF protein in over expression of the doubtful organization is expected to provide the medical evidence for the early diagnosis and treatment of nasopharyngeal carcinoma intervention, the prognosis judgment and the guidance for clinical and targeted drugs research.Research purposeTo test stimuli response of key proteins, and PCDGF gene expression and biological behavior of nasopharyngeal carcinoma (NPC) in nasopharyngeal carcinoma tissue, explore HIF1-α and PCDGF value in the diagnosis and treatment of nasopharyngeal carcinoma (NPC), the evaluation of HIF-1α and PCDGF gene for the prognosis of patients with nasopharyngeal carcinoma (NPC), the value and significance.Research methodsApplication of immunohistochemical technique, using indirect immunofluorescence staining Method of Double (Double Layer Method) of78cases of nasopharyngeal carcinoma tissue specimens and20cases of normal nasal pharyngeal mucosa specimens of immunohistochemical staining, detection of HIF-1a and PCDGF gene expression in it, and on this basis further analysis and biological behavior of nasopharyngeal carcinoma (NPC), and to explore both in terms of diagnosis, treatment and prognosis of nasopharyngeal carcinoma (NPC) assessment of value.The results of the study1.HIF1-α anud PCDGF in nasopharyngeal carcinoma tissues were expressed; According to the2008clinical staging stage nasopharyngeal carcinoma (NPC), guangzhou Ⅰ, Ⅱ period in patients with HIF-1α and high PCDGF gene expression rate is much lower than Ⅲ,Ⅳ period in patients with HIF-1α and PCDGF gene expression rate high, the differences between the two groups have statistical significance;2.Who have a relapse after treatment and in patients with cervical lymph node metastasis in nasopharyngeal carcinoma tissue HIF-1α and PCDGF positive expression rate (including+/+++) is significantly higher than and neck lymph node metastasis in patients with no recurrence;3.HIF-1α and PCDGF expression was recurrence and distant metastasis rate is significantly higher than the negativ.The expression level and PCDGF HIF-1gene in patients with sex, age have no statistical significance;4. HIF-1α and PCDGF is positive, one-year survival rate negative there was no statistically significant difference, but the3year survival rate of a and PCDGF HIF-1gene is positive, negative;5. HIF-1α and PCDGF expressed in common:①in terms of lymph node metastasis, PCDGF (+++)/α (+) and PCDGF HIF-1(++) or less/a (+) or PCDG HIF-1(+++)/HIF-1α (-) no difference (P=0.061>0.05), PCDGF (++) or less/a (-) and PCDGF HIF-1(+++)/HIF-1α (+), PCDGF (++) or less/a (+) or PCDG HIF-1(+++)/HIF-1α(-) differ (P=0.011<0.05);②As for the blood line of distant metastasis, distant metastasis: PCDGF (+++)/α (+) and PCDGF HIF-1(++) or less/α (+) or PCDG HIF-1(+++)/HIF-1α (-) no difference (P=0.29>0.05), and C PCDGF (+++)/α (+), PCDGF HIF-1(++) or less/HIF-1α (+) or PCDG (+++)/HIF-1α (-) no difference (P=0.156>0.05);③T (Tumor Node Metastasis staging, PCDGF (+++)/HIF-1α (+)\PCDGF (++) or less/a (+) or PCDG HIF-1(+++)/α (-)\PCDGF HIF-1(++) or less (++)/HIF-1α (-) the distribution of the three groups of different, have a plenty of is not all the same.6.Nasopharyngeal carcinoma tissues PCDGF a protein expression was positively correlation with HIF-1,=0.765,P<0.01)7.The prognosis of patients with nasopharyngeal carcinoma (NPC) and the system only associated with and without recurrence after treatment and neck lymph node metastasis, Cox regression equation is: h(t,x)=hO e x p (t)(0.7412*3c3b-1.0077+0.1603+0.3585*4*5x8c+0.2703+0.7019*9). The research conclusionHIF-la and PCDGF expression in nasopharyngeal carcinoma tissues may involve the emergence and development process of nasopharyngeal carcinoma (NPC), HIF-la and PCDGF expression levels of detection possible diagnosis and staging of nasopharyngeal carcinoma and the curative effect judgment has important value.HIF-lα and PCDGF protein high expression is closely related to the2008guanzhou nasopharyngeal carcinoma clinical staging, Ⅰ, Ⅱ period in patients with HIF-1α and PCDGF protein expression rate and high Ⅲ, IV period in patients with HIF-1α and PCDGF high protein expression rate as the difference was statistically significant; there is a statistical significance displaying that HIF-1α and PCDGF protein expression may be closely related with the clinical stage of nasopharyngeal carcinoma (NPC), may occur in nasopharyngeal carcinoma, play an important role in the process of development.HIF-lα and PCDGF proteins or license as candidates for the nasopharyngeal carcinoma occurrence and metastasis suppressor genes; Can be used as molecular markers of nasopharyngeal carcinoma prognosis evaluation; And probably as a potential therapeutic targets.