| Environmental endocrine disruptors (Endocrine disrupting chemicals, EDCs) monitoring and evaluation of ecological toxicology effects have become the focus issues in the fields which include ecology and agriculture and soil science and so on. Nonylphenol (NP) is a kind of alkyl phenolic compounds, have certain environmental estrogen effect, stems mainly from industry, textile, medicine, detergent and cosmetics substances such as central Africa ionic surfactant nonylphenol polyoxyethylene ether decomposition or degradation products. NP has the characteristic such as easy to enrichment, refractory, can through the water, soil and air into the animal and plant body, enrichment and magnified through the food chain. With the construction of energy chemical industry base in northern shaanxi, phenol organic matter on the soil and the toxicity of aquatic organisms normal growth and development cannot be ignored. The test studied on mature and juvenile Misgurnus anguillicaudatus were exposed to NP, the toxicity effect of mature and juvenile fish、the damage of gill and liver, blood cell micronucleus effect, vitellogenin content were analyzed, the results are as following:1. The acute toxicity effect of nonylphenol on mature and juvenile Misgurnus anguillicaudatusNonylphenol on Misgurnus anguillicaudatus 24,48,72,96 h of lethal concentration were 3.620,4.225,5.044,6.648 mg/L, safe concentration was 0.870 mg/L; Nonylphenol on juvenile 24,48,72,96 h of lethal concentration were 3.722,2.939,2.466,2.175 mg/L, safe concentration was 0.550 mg/L.2. Gill and liver histologic damage of mature and juvenile Misgurnus anguillicaudatus exposed to NPHistological observation showed that high concentrations nonylphenol processing small piece of gill cells of mature Misgurnus anguillicaudatus damaged badly, columnar cells and epithelial cells fell down. liver cells appeared vacuoles、swelling、nuclear fall off phenomenon. liver in vacuoles, fuzzy boundaries; while small piece of gill cell of juvenile Misgurnus anguillicaudatus fell off but the damage effect was more serious than mature Misgurnus anguillicaudatus; liver cells in vacuoles, swelling, nuclear fall off etc. the results showed that the nonylphenol belongs to highly toxic pollutants, toxic effects on juvenile was along with the increased concentration of accumulation in the body, action time and enhance, and can cause gill tissue was badly damaged. Compared with fish, juvenile of SC value smaller, gills and liver injury is more serious.3. Oxidase activity of liver tissue and VTG content of serum of juvenile Misgurnus anguillicaudatus exposed to NPJuvenile Misgurnus anguillicaudatus exposed to low concentration NP in 15 d, SOD and CAT activity along with the increment of concentration of NP, peaked at 0.188 mg/L, then a downward trend. Prompt NP to juvenile Misgurnus anguillicaudatus with high toxicity, toxic effect with the increase of mass concentration and stress increased with the extension of time; NP can affect juvenile fish liver SOD and CAT activity, along with the increase of mass concentration after first induced inhibition performance, resulting in oxidative damage. content of juvenile Misgurnus anguillicaudatus’s serum were extremely significantly higher, and along with the increment of concentration, NP of male juvenile fish serum vacations has significant entrainment, and with the increase of mass concentration induced enhancement.4. Genetic toxicity of mature Misgurnus anguillicaudatus exposed to NPNP poison mature Misgurnus anguillicaudatus for 24,48,72,96 h, get four time of micronucleus rate of peripheral blood were 1.35,2.49,3.80,2.49‰, respectively. Compared with the control group of 24 h,48 h micronucleus rate were significantly (p< 0.05),72,96 h micronucleus rate were very significant (p< 0.01). The above data showed that NP can produce genetic toxicity on Misgurnus anguillicaudatus, micronucleus rate will increase with concentration of NP quality and increased with the extension of time. |
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