Effects Of Coenzyme Q10in The Liver Of Rat Of Preeclampsia-like Symptoms

Part I Establishment of preeclampsia-like rat model and evaluation of thetherapeutic effects of Coenzyme Q10ObjectiveThe purpose of this study was to establish preeclampsia-like model in pregnant ratsby using nitric oxide synthase (NOS) inhibitor-L-nitro-arginine-methylester (L-NAME),and investigate the treatment of coenzyme Q10from blood presure, urine protein, andother aspects of the outcome in the offspring of the preeclampsia-like rats.MethodsThirty pregnant SD rats were randomized equally divided into three groups andreceived subcutaneous saline injecfion(normal pregnancy group, NP group),125mg/kgL-NAME injection to induce preeclampsia symptoms(distilled water group, DW group),or L-NAME with60mg/kg CoQ10gavage(CoQ10group). Detect the blood pressure,urine protein, ALT and AST.Results①Blood pressure: No significant difference was found between the three groups inblood pressure of day10(P>0.05). The blood pressure rised respectively on D15inmodel group (P<0.05), also were higher than NP group, respectively(P<0.05). DWgroup maintained blood pressure at a high level on D21, no significant difference wasfound as compared to D15(P>0.05). The blood pressure in CoQ10group on D21decreased as compared to D15.It was lower as compared to DW group but increasedas compared to NP group (P<0.05).②urine protein: No significant difference was found between the three groups in24hours urine protein of D10.24hours urine protein were increased in the model groupson D15as compared to pre-pregnancy (P<0.05), and also higher than NP group(P<0.05).24hours urinary protein was higher in DW group on D20as compared to NPgroup. It was less in CoQ10group than Dw group and higher than NP group(P<0.05).③Serum transaminases: ALT and AST were significantly higher in DW group than NPgroup. ALT and AST were significantly lower in CoQ10group as compared with DW group but still elevated in NP group (P<0.05).④Offspring outcomes: Rat fetuses and fetal weight were lower in DW group than NPgroup. Rat fetuses and fetal weight wree significantly raised in CoQ10group thanDW group and lower than NP group. Placental weights were lower in model groupthan NP group (P<0.05). Placental weights were higher in CoQ10group than DWgroup (P<0.05).ConclusionsIt could fully simulate the main performance of preeclampsia by using L-NAME125mg/kg/d subcutaneous, and successfully established model of preeclampsia-like rat.CoQ10could be effective in improving the performance of preeclampsia-like rats andimproved offspring outcome. PartⅡ The effect of coenzyme Q10in the liver of rats of preeclampsia-likesymptomsObjectiveTo investigate the protective effect of coenzyme Q10in the liver of rats ofpreeclampsia-like symptoms.MethodsFifty pregnant SD rats were randomized equally into5groups and receivedsubcutaneous saline injecfion(control group)，125mg/kg L-NAME injection to inducepreeclampsia symptoms(DW group)，or L-NAME with60mg/kg CoQ10gavage(CoQ10group), or L-NAME with60mg/kg CoQ10gavage and120mg/kg Magnesium injection(CM group), or L-NAME with120mg/kg Magnesium injection (Mg group). Detect theblood pressure, urine protein and ALT, AST values, MDA, SOD and GSH-PX activity inliver. Western-blot assay liver Caspase-3, Bcl-2and Bax protein expression.ResultsCompared with DW group, blood pressure, urinary protein, ALT and AST weresignificantly lower in CoQ10group. GSH-PX and SOD activity were lower in modelgroup than normal pregnant group, pairwise comparison, the differences were statisticallysignificant. Compared with DW group, CoQ10group and Magnesium group were higher,respectively. CM group, DW group and Mg group were higher, the differences werestatistically significant; CoQ10group with mildly elevated, but the difference was notstatistically significant. GSH-PX and SOD activity were significantly increased in Mggroup than DW group. Liver MDA level in NP group was (5.49±0.32) nmol/mg protein,and it was statistically higher in model group than NP group. DW group, CoQ10group,CM group and Mg group were (9.38±0.51) nmol/mg protein,(6.80±0.32) nmol/mgprotein,(6.86±0.45) nmol/mg protein and (7.73±0.28) nmol/mg protein. The differencewas not statistically significant between CoQ10group and CM group. The differenceswere statistically significant between any two remaining groups. As compared with othergroups, the protein expression of activated Caspase-3, Bax in the livers were significantlydecreased in CoQ10group, Mg group and MG group compared with DW group(P＜0.05), they were significantly increased compared with NP group(P＜0.05). The proteinexpression of Bcl-2was significantly increased in CoQ10group, Mg group and MG group compared with DW group(P＜0.05), and it was significantly decreased comparedwith NP group(P＜0.05).ConclusionsCoQ10could effectively improve the liver functions in PE-like rats and markedlydecrease the oxidative stress and apoptosis in the livers. Anti-oxidative stress andenhanced expression of Bcl-2and suppressed expression of Bax may be involved in theanti-apoptotic effect of CoQ10.