| Syringa wolfii C. K. Schneid. belongs to the Oleaceae, Syringa deciduous shrub,Syringa wolfii C. K. Schneid. because of its unique color (purple or lavender), longflowering period, in the north of China, it’s widely used as an ornamental plant. Thethesis is mainly divided into seven parts to conduct a systematic research andpreparation of Syringa wolfii C. K. Schneid.. The paper focuses on the research ofchemical composition, quality evaluation, antioxidant, hepatoprotective activity studyof the branch from Syringa wolfii C. K. Schneid., which provides a reference basis forthe development and utilization of resources in Syringa wolfii C. K. Schneid..The author of this paper not only makes a systematic exposition of the reports onthe main chemical components and pharmacological effects of Syringa, but alsoSyringa wolfii C. K. Schneid., which provides the background of research andreference for the further study on the chemical constituents and pharmacologicaleffects of clove in this plant, Syringa wolfii C. K. Schneid..The study conducted the crude separation on Syringa wolfii C. K. Schneid. ofthis study using the system solvent method, which obtained the chloroform, ethylacetate and n-butanol layer, and done carries on the separation by columnchromatography and Thin Layer Chromatography, and so on, and which obtained8chemical compounds, and6compounds were identified at the end, it’s dimethylterephthalate,3-hydroxy-Ursane-12-ene, ursolic acid,4-(2-hydroxyethyl)-phenol,D-mannitol and syringin. Further more, ursolic acid, D-mannitol and syringin areobtained for the first time. Because the contents of3-hydroxy-Ursane-12-ene andsyringin are fairly high, which will provide protophase theoretical reference for thegathering of this two compounds from Syringa wolfii C. K. Schneid..The study extracted essential oil by steam distillation, and identified the chemicalcomponents by GC-MS method, and detection of88peaks were separated bycapillary chromatographic, finally,69compounds were identified, and2-methyl-5-(1-methyl ethyl)-1-cyclopentene alcohol(14.16%) was with the high contentamong them. The analysis of the result show that sesquiterpenes of the branch fromSyringa wolfii C. K. Schneid. accounted for the largest proportion, the content of totalsesquiterpene compounds are as high as39.04%, the total content of monoterpenecomponents reached15.48%, the highest content component of essential oil ofSyringa wolfii C. K. Schneid. is monoterpene compounds. Therefore, the essential oilof Syringa wolfii C. K. Schneid. possesses high content of terpene compounds.This research uses the HPLC method to detect the content of syringin as index,the content of syringin in different parts of Syringa wolfii C. K. Schneid. was determined, assays include feasibility test, precision test, reproducibility and RSDindicated that the experimental data is good, the results showed that: peel of Syringawolfii C. K. Schneid. contains the highest content, and branches does fairly high.On this basis, the experiment also determined the content of syringin in differentharvest period of branch from Syringa wolfii C. K. Schneid. The results showed that:the content of syringin in different harvest period of branch from Syringa wolfii C. K.Schneid. is different, in the October12th, the content of syringin is up to0.723%, andthe lowest in July2nd was0.047%.The results suggest that the best collecting time ofSyringa wolfii C. K. Schneid is mid-October.The determination of flavonoids of branches from Syringa wolfii C. K. Schneid.in different harvest period performed by UV spectrophotometry, and polysaccharidecontent was determined by anthrone-sulfuric acid colorimetric method for thedetermination of branch from Syringa wolfii C. K. Schneid. In different harvestperiod, results show that: two peaks of content appeared from March to October, thehighest content of flavonoid is in March, it;s8.52%, and peak of the content appearedagain in mid October, it’s7.93%; however, the content of polysaccharide is thehighest from mid September to mid October content, which is up to12.67%or12.28%. So take highest harvest time of flavonoids and polysaccharide into account,the best harvest period of branches from Syringa wolfii C. K. Schneid. should be midMarch or mid October.Comparison of antioxidant capacity of this study was determined by DPPHmethod, ABTS method, ferric ion reduction method and total phenol method(fourvitro antioxidant methods) for various layers of branches of Syringa wolfii C. K.Schneid., and results evaluating and screening the best antioxidant layers, the resultsshowed that ethyl acetate layer, the chloroform layer and n-butyl alcohol layer in thefour oxidation method exhibit good antioxidant activity, and in a concentrationdependent, and the ethyl acetate layer performs the best approaching the BHT, whichshowed that, the phenolic compounds of ethyl acetate layer with high content, withenhanced effect on the activity.In this study, alcohol induced liver injury of mouse model was used as theresearch object, the mice were given different doses of water extract of branch fromSyringa wolfii C. K. Schneid., comparison with negative group, positive group andcontrol group on mice activities, the liver index, serum ALT and AST and liver MDAcontent changes of chemical indicators, results showed that: water extract of branchfrom Syringa wolfii C. K. Schneid.of each dose group can make these indexesreduced at each does, at the same time, compared with the negative group, high dosegroup of branch from Syringa wolfii C. K. Schneid. can significantly reduce the liverindex and serum ALT in mice, the activity of AST, but not to change the content ofMDA in liver tissue, the results showed that the water extract of branch from Syringawolfii C. K. Schneid. in the prevention of alcoholic liver injury in mice was not dosedependent.The innovation of this paper: 1. This study on the extraction and separation of chemical constituents of branch fromSyringa wolfii C. K. Schneid. was for the first time, which provided a theoretical basisfor further development and application.2. Study on the chemical constituents of volatile oil of branch from Syringa wolfii C.K. Schneid. of the study, results showed that the volatile oil of branch from Syringawolfii C. K. Schneid. contains alkenes, alcohols, aromatic hydrocarbons, ketones,alkanes and other ingredients.3. The study based on previous study on to senior, syringin, flavonoids andpolysaccharide content were used as the indexes using three methods to determine theoptimum harvest time of branch from Syringa wolfii C. K. Schneid., to provide thetheoretical basis for resource gathering and application of Syringa wolfii C. K.Schneid..4. Different doses of total extracts of branch from Syringa wolfii C. K. Schneid. werestudied in alcoholic liver injury in mice, total extract branch from Syringa wolfii C. K.Schneid. has protective effects on alcoholic liver injury in mice, this study canprovide a new source of hepatoprotective medicine for the research in the field ofliver disease. |
PDF Full Text Request