Renal Physiologic Pulsatile Perfusion Preservation And Function Impairment Research

Organ transplantation is one of the effective measures to treat multiple organ failure inpatients with life. With the improvement of scientific and medical technology, the type oforgan transplantation increases.For many reasons, the number of organ transplantationpatients has increased dramatically, resulting in the number of transplantation donors ismush less than patients. Many cardiac death donors and marginal donors now begin to beused in a large number of clinical transplantation, which requests the traditional kidneypreservation techniques.In this situation, we designed a multi-parameter detection of kidneybiomimetic pulsatile perfusion preservation system,the results are as follows：1. we developed a kidney biomimetic pulsatile perfusion preservation system.Thesystem was driven by a dc motor to perfuse kidney, which is more convenient and stablethan traditional ones；2. The system can be setted different perfusion pressure and pressure pulsationwaveformaccording to the size of the kidneys and ages to simulate in vivo environment, atthe same time reduce reperfusion injury.3. The system is designed with multi-parameter sensor system: detecting in real timethe ambient temperature in the range of perfusion to-5℃to45℃; real-time detection ofchanges in the pressure within the range of0mmHg to100mmHg perfusion tubing;detection whether there are bubbles in the perfusion tubes, if so, promptly remove airbubbles.4. We experiment with the system on both continuous and pulsatile perfusion.Experiments. Experimental results show that changes in kidney function can be divided intotwo stages:0-2hours cryogenic stage adaptation of the kidneys,2-24hours of steady stagerenal function, renal function smoothly at this stage, but with the perfusion time increasingfunction showed a slow downward trend.5. We take renal cortex tissue samples and make the slice. Observe structure was undera microscope. Results shows that the system we design has effective functionon protectingrenal tubular structure.the system can be set to a different value according to renal perfusion pressure ofdifferent sizes, ages, while matching different waveforms perfusion pressure changes, toensure that the effect of perfusion, while reducing reperfusion injury； 5. the system cleverly designed perfusion buffer chamber pipes and bubbles, thebubble detector can detect down to ensure that no air bubbles up into the kidney perfusion,the greatest degree of reduced renal reperfusion injury.Given the time constraints, the main work of this project is to design and develop thiskidney biomimetic pulsatile perfusion preservation systems, and do some prime experimentwith this system to verify its advantages in terms of renal perfusion preservation.Kidneypreservation techniques and dynamic renal dysfunction have a very significant meaning.Wewill further explore in the next.