The Hangover Liver Efficacy Of Citrus Grandis’tomentosa’ And Its Health Food Research

ObjectiveThe famous-region drug of Guangdong HuaZhou called Citrus grandis ’Tomentosa’has dispel the effects of alcohol efficacy, this paper is rich in the active components of flavonoids compounds in Citrus grandis’Tomentosa’in the dispel the effects of alcohol efficacy-research and development of oral liquid dosage forms, for the unique medicinal materials provide a scientific basis for comprehensive exploitation and utilization of resources.MethodsWater and diacolation method were used respectively to extract flavonoids in pummelo peel, the application of ultraviolet spectrophotometer determination of flavonoids content in Citrus grandis’Tomentosa’, UPLC determination of the content of naringin in Citrus grandis’Tomentosa’extract, Finally to extract rate, naringin extraction yield and cost to measure the pros and cons of two extraction methods. For different batches of Citrus grandis ’Tomentosa’, in order to ensure the quality of medicinal materials. Due to the low solubility total flavonoids in Citrus grandis ’Tomentosa’this research adopts the hydroxypropyl-β cyclodextrin inclusion, for the various influence factors of the inclusion reaction (material mole ratio, inclusion temperature, time, stirring speed inclusion) to test, with inclusion rate and the drug as an index, find out main influence factors, the best inclusion process and screened by orthogonal experiments.The resulting total flavonoids of Citrus grandis’Tomentosa’ clathrate materials be added to taste deployment solution, and through the filter in removing impurity, potting mixture liquid dosage forms, leak detection, sterilization, establish oral liquid dosage form drug quality standard including the main content, microbial health, the main drug identification, pH value, relative density, and6months for the long-term stability of the test. With28°double steamed wine drunk dose to test in mice, at the same time use drink and drugs (from ingesting experiment), wine (against drunk) after medicine, behavioral science experiments in mice of test the efficacy of oral liquid therapy efficacy.ResultsThe optimum process for20times of water, water extracting time3h, extraction times2times; Diacolation optimum process for the method of orthogonal design:diacolation6times with70%ethanol, ethanol immersion time of24h, the experimental verification, parallel determination of3,200g hair orange medicinal materials available water naringin23.97±0.03g, extract rate was63.21%. Diacolation method available naringin28.99±0.03g, extract rate of46.43%.Content determination, for many kinds of pummelo peel orange light quality is lower than the hair orange, mainly reflects the opposition sumac glycosides content above, in the hair orange varieties (big tea ridge, western, Chinese brake, money belly. Yellow fruit, Liu Fu) from the content of naringin, false western optimal; From the point of wild sumac glycosides, yellow wool fruit optimal. Together, the western, money belly, yellow fruit is good varieties.Citrus grandis’Tomentosa’fingerprint study showed that on41batch of different cultivars and orange medicine fingerprint analysis, calibration14common peak, similarity in0.938-0.998, the first peak of naringin and the second peak of wild sumac glycosides.2013years collected samples of Citrus grandis’Tomentosa’, respectively, in the large tea naringin Ridge, fake Western, Pteris, money belly, yellow velvet fruit, Lu Fu, light green in content:(102.676+28.103,115.788±14.153,134.996±37.378,101.589±18.435,116.442±23.217,104.602±18.050,92.674±32.767) mg/g, respectively, in large rhoifolin Cha Kwo Ling, fake Western, Pteris, money belly, yellow velvet fruit, Lu Fu, light green in content:(2.595±0.744,3.458±2.621,1.795±0.870,3.278±0.556,7.613±0.717,5.887±1.984,0.506±1.894) mg/g, respectively, in the large tea naringenin Ridge, fake Western, Pteris money belly, yellow velvet fruit, Lu Fu, light green in content:(0.591±0.173,0.615±0.291,0.624±0.186,0.661±0.228,0.544±0.128,0.554±0.116,0.628±0.110) mg/g, apigenin were big tea Ridge, fake Western, Pteris, money belly, yellow velvet fruit, Lu Fu, light green in content:(0.051±0.023,0.043±0.034,0.053±0.014,0.086±0.003,0.050±0.033,0.049±0.022,0.073±0.022)Results show that the best process for the inclusion of total flavonoids from Citrus grandis’Tomentosa’:HP-beta-CD with Citrus grandis’Tomentosa’flavonoids mole ratio of1:1, anhydrous ethanol and water volume ratio of1:1, stirring time is2h, under the optimal process conditions, the maximum combined rate can reach (77.1±6.4)%, the largest drug loadings can reach (14.0±0.3)%, through a microscope,differential scanning calorimetry, infrared spectroscopy for the identification of clathrate, determine the formation of inclusion compound total flavonoids pummelo peel.The experiment showed that Citrus grandis’Tomentosa’flavonoids inclusion solution1ml add2ml70%honey water, xylitol is0.2g and0.125g citric acid, with the capacity to10ml of the purified water can achieve the best taste.Quality standard for the pH value of Citrus grandis’Tomentosa’ Oral hangover should be controlled between1.80～1.90, the relative density should be not less than1.09, thin layer chromatography should check out the naringin, wild sumac nucleoside composition, oral liquid in the bottle containing naringin shall not be less than3mg.0.20ml/10g dose administered to mice by gavage, only one mouse appears on top of the phenomenon, the effect of righting reflex disappears into sleep after waking back to normal, showing the degree of liquor did not reach the desired test concentration.0.25ml/10g dose group had six top phenomenon occurs, loss of righting reflex, but only five will enter normal to sleep after waking up, the test can provide sample size is still not enough.0.30ml/10g dose group appeared drunk nine top phenomenon,7goes to sleep after waking up, a death, as opposed to0.35ml/10g died4,0.4ml/10g died7is better, so this experiment to determine the use of28±double-distilled alcohol concentration caused by drunken dose mice0.30ml/10g.After the first "drug","wine" anti-drunk trials, oral high, medium and low dose group sleep latency times were:(39.32±3.02) min,(28.52±2.90) min,(23.14±2.42) min, blank group, positive drug group sleep latency times were:(21.56±3.66) min,(53.06±4.29) min, oral high, medium and low dose sober times were:(285.40±24.16) min,(328.58±23.12) min,(333.50±18.44) min, the control group, positive drug group sober times were:(334.80±29.65) min,(201.33±15.66) min.After the first "wine","medicine" hangover trials, oral high, medium and low dose groups were sobering time:(327.09±31.13) min,(346.11±23.12) min,(352.50±23.54) min, the control group, positive drug group sober time was:(368.27±44.20) min,(281.20±35.23) min.Cling test, oral high, medium and low dose groups cling times were:(279.4±22.1,273.6±10.6,265.1±18.4) s, the control group, positive drug group clings times were:(254.4±31.5,316.0±25.3) s.Citrus grandis’Tomentosa’oral high-dose group after the first "medicine","wine" hangover was14.76%in advance,"medicine" first18%ConelusionFrom the extraction rate and the rate of the active ingredient cream naringin, the percolation method is superior to conventional heat reflux extraction, and more in line with the large industrial production, energy saving and environmental protection, For many varieties of Citrus grandis ’Tomentosa’ were determined content, the quality is below the light orange hair orange, mainly opposition sumac glucosinolate content above, in the hair orange varieties (large tea Ridge, fake Western, Pteris, money belly. Yellow velvet fruit, land Fu) from naringin, the fake Western optimal; from rhoifolin view, the best yellow velvet fruit. Taken together, the fake Western, money belly, yellow velvet fruit are better varieties.Total flavonoids of Citrus grandis ’Tomentosa’ inclusion processresearch Hydroxypropyl-β-cyclodextrin inclusion of well orange flavonoids and increase its solubility, stability, resulting clathrate quality, consistent UV wavelength of maximum absorption of total flavonoids and non inclusion will not interfere.Research on the quality of Citrus grandis ’Tomentosa’ oral hangoverTotal flavonoids of Citrus grandis ’Tomentosa’ through inclusion, its taste is still bitter, add honey, citric acid, xylitol, the better masked their bitter into sweet and sour, and no sugar recipes for high blood sugar patients taking. The resulting process is stable, after examining the long-term stability, clarity and microbial health indicators are in compliance.Preliminary study of the efficacy of Citrus grandis ’Tomentosa’ oral hangoverOral health of Citrus grandis ’Tomentosa’ for acute alcoholism has a certain effect, can quickly relieve drunk hangover and discomfort arising from the process, and prevention is better than drunk hangover effect.