Study On The Chemical And Biological Activity Of Rubia Cordifolia L. Collected From Henan Province

This dissertation is composed of three chapters. The first chapter included studies on the isolationand identification of compounds from Rubia cordifolia L. The second chapter studied the antioxidant andcoagulant activity of the extracts and compounds from R. cordifolia L. The third chapter summarized theprogress of chemical constituent of Rubia genus.Chapter1. The chemical constituents of Rubia cordifolia L.The chemical constituents from the R. cordifolia L were studied by column chromatography.Twenty-seven compounds were isolated and twelve were identified as1-hydroxy-2-methyl-9,10-anthraquinone (1),1,4-dihydroxy-2-methylanthraquinone (2), palmitic acid (3),tricosanoic acid (4), ω-hydroxypachybasin (5), ursolic acid (6), oleanolic acid (7), rubilactone (8),β-sitosterol (9), cordifoliol (10),2,3-dimethyl-1,4-dihydroxyanthraquinone (11)1,3-dihydroxy-2-ethoxymethyl-9,10-anthraquinone (12). Compound5and11were isolated from Rubia genus for thefirst time.Chapter2. The biological activity of Rubia cordifolia L.DPPH, ABTS, and FRAP assays were used to screen the antioxidant activity of R. cordifolia L.The study discovered that the initial screening inhibition rate of EtOH extract less than50%, it showed no DPPH radical scavenging ability. In ABTS method, its radical scavenging ability had a dose-dependent,with the increase of its concentration, the ABTS free radical clearance rate reinforce. In FRAP method, theEtOH extract had a certain reduction ability of Fe3+, but less than that of the positive reference substance.The EtOH extracts from R.cordifolia showed ordinary antioxidant activity.Coagulant activity of the extract and different compounds from R. cordifolia assayed in vitro.The EtOH extracts from R. cordifolia showed anticoagulant effect.1,4-dihydroxy-2-methylanthraquinone,rubilactone and1,3-dihydroxy-2-ethoxymethyl-9,10-anthraquinone showed procoagulant effect.Chapter3. Advance on the Chemical Components of RubiaGenusThe progress of chemical constituent of Rubia genus were summarized.The genus Rubia (Rubiaceae), including70species, is widely distributed in Europe, Asia,Africa, America, and a few in Mediterranean. There are36species and2varieties in China(Editorial Committee of Flora of China, Chinese Academy of Sciences,2005). R. cordifoliais known as Chinese traditional medicine for the treatment of haematemesis, non-traumatichemorrhage, uterine bleeding, stasis amenorrhea, traumatic bleeding, arthralgia, painfulswelling from knocks (State Pharmacopoeia Commission,2010), especially shownsignificant bioactivities for the treatment of cardiovascular and cerebrovascular diseases(Zhang et al.,2008). Phytochemical research showed that anthraquinones, naphthoquinones and terpenes were main constituents in the genus (Li P et al.,2013; Zhang et al.,2008).Pharmacological investigations had demonstrated that the extract of R. cordifolia hadsignificant bioactivities such as antitumor (Itokawa et al.,1983), antimicrobial (Wang XJ etal.,2012), immunoregulation (Su XL et al.,1992), anti-inflammatory antirheumatic (Xu LZet al.,2002) and antioxidant (Kang WY et al.,2006). Different environmental or ecologicalconditions can cause differences in plant active ingredients (Wang ZY,2003). In search ofnew bio-active resources of R. cordifolia collected from Henan Province and provide atheoretical basis for the further development and utilization, a systematic study on thechemical constituents and their biological activity are reported herein.