Clinical Study On The Effect Of Yiqi Sanjie Detection On Treating The Kidney Deficiency And Blood Stasis Type Of Adenomyosis And Its Effect On The Immune Balance Of Th17/Treg Cells

Objective:1. To observe the changes of dysmenorrhea, menstrual quantity, ShenXu concomitant symptoms, traditional Chinese medicine syndrome, uterine volume, serum CA125value and serum hormone levels before and after the treatment on the kidney deficiency and blood stasis type of adenomyosis in order to evaluate its clinical effects and provide a clinical basis for chinese medicine treatment of adenomyosis.2. To observe the influence of YiQi SanJie Decoction on serum complementary C3, C4, serum immunoglobulin IgG, IgM, population of peripheral blood Thl7cells and Treg cells and related cytokines, such as IL-6, IL-10, IL-17, TGF in order to discuss the curative effect of YiQi SanJie Decoction on the kidney deficiency and blood stasis type of adenomyosis, and future explore its probable mechanism.Methods:1. Seventy cases who were diagnosed as the kidney deficiency and blood stasis type of adenomyosis were randomly assigned into two groups. Fifty cases for the Chinese medicine treatment group were treated with YiQi SanJie Decoction. Twenty cases for the Mirena group were treated with the LNG-IUS. The both two groups were treated for3months. The changes of dysmenorrhea, menstrual quantity, ShenXu concomitant symptom scores, traditional Chinese medicine clinical syndrome scores, uterine size, the serum CA125values and serum hormone levels before and after the treatment were observed.2. Fifty cases of healthy women who took normal physical examinations were included into the normal control group. The levels of serum complementary C3, C4, immunoglobulin IgG, IgM, peripheral blood Th17cells, Treg cells and related cytokines, such as IL-6, IL-10, IL-17, TGF were evaluated. The above mentioned immune indexes of the traditional Chinese medicine both before and after the treatment were compared with the normal control group.Results:1. Clinical study:(1)Dysmenorrhea evaluation:The dysmenorrhea symptom scores after the treatment were significant different from those before the treatment in the both treatment groups(P<0.01).There was significant difference between the dysmenorrhea symptom scores of the two treatment groups after the treatment(P<0.01);(2)Menstrual quantity evaluation:The menstrual quantity scores after the treatment were significant different from those before the treatment in the both treatment groups(P<0.01). There was no significant difference between the two treatment groups after treatment (P>0.05);(3) Traditional Chinese medicine syndrome evaluation:The ShenXu concomitant symptom scores after the treatment were significant different from those before the treatment in the both treatment groups(P<0.01), There was significant difference between the dysmenorrhea symptom scores of the two treatment groups after the treatment (P<0.05); The traditional Chinese medicine syndrome scores after the treatment were significant different from those before the treatment in the both treatment groups(P<0.01). There was no significant difference between the two treatment groups after treatment (P>0.05).(4)The uterine volume of Chinese medicine group after the treatment was significant different from those before the treatment(P<0.05), the uterine volume of Mirena group after the treatment was not significant different from those before the treatment(P>0.05). There was no significant difference between the two treatment groups after treatment(P>0.05).(5)The serum carbohydrate antigen CA125values of Chinese medicine group after the treatment was significant different from those before the treatment(P<0.01), the serum carbohydrate antigen CA125values of Mirena group after the treatment was not significant different from those before the treatment (P>0.05), There was no significant difference between the two treatment groups after treatment(P>0.05).(6) There was no significant difference between the two treatment groups of the serum PRL, T3, T4levels before and after treatment(P>0.05);2. Experimental study: Compares with the control group, the population of Th17cells in the whole blood was significantly increased(P<0.05), while the population of Treg cells was significantly decreased(P<0.05), resulting in significant increase of the Th17/Treg ratio in the patients with adenomyosis(P<0.05). After treatment with YiQi SanJie Decoction, the population of Thl7in whole blood was significantly reduced(P<0.05), with the population of Treg cells increased(P <0.05), and the ratio of Th17/Treg cells decreased in patients with adenomyosis(P<0.05). After treatment, the populations of Th17and Treg cells as well as the Th17/Treg ratio of the YiQi SanJie Decoction group were not significantly different from those of the control group. YiQi SanJie Decoction did not significantly affect the levels of serum complementary C3, C4, immunoglobulin IgG, IgM, peripheral blood Th17cells, Treg cells and related cytokines, such as IL-6, IL-10, IL-17, TGF(P>0.05).Conclusion:Both YiQi SanJie Decoction and LNG-IUS improved dysmenorrhea, menstrual quantity, ShenXu concomitant symptoms, traditional Chinese medicine syndrome of the stagnancy of Qi and blood stasis type of adenomyosis. LNG-IUS improved dysmenorrhea better than YiQi SanJie Decoction, YiQi SanJie Decoction improve ShenXu concomitant symptoms better than LNG-IUS. YiQi SanJie Decoction has certain clinical curative effect on the treatment of the kidney deficiency and blood stasis type of adenomyosis. Its probable mechanism may be that it can obviously improve the body’s immune imbalance.