| Objective:To determine whether sevoflurane preconditioning protects lung injury induced by ischemia-reperfusion in rats and explore the mechanisms involved, which offers the experimental and theoretical evidence for the choice of anesthetics in clinical practice. Methods:fifty-four male SD rats weighing250-320g were randomly divided into3groups (n=18each):sham operation group (group S):the hilum of left lung was isolated but not clamped; lung ischemia-reperfusion (I/R):the hilum of left lung was occluded for45min and followed by reperfusion; sevoflurane preconditioning group (group SP):2.1%sevoflurane inhalation for30min before lung ischemia-reperfusion injury. Six rats per group were randomly sacrificed at30min,60min,120min of reperfusion, respectively. The lung tissues were taken for measurement of wet/dry weight (W/D) ratio and determination of the contents of MPO by colorimetric method and the expression of ACE mRNA by RT-PCR and histological examination. Results:Compared with group S, the W/D ratio of lung, content of MPO and the expression of ACE mRNA were significantly increased in group I/R and SP (P<0.05). With the extension of reperfusion time the W/D ratio of lung, content of MPO and the expression of ACE mRNA in both group I/R and group SP were increased. However, the W/D ratio of lung, content of MPO and the expression of ACE mRNA were significantly lower in group SP than those in group I/R (P<0.05). Histological examination showed that the injury of alveolar structure was alleviated in group SP relative to group I/R. Conclusion: Sevoflurane preconditioning might protect lung injury induced by ischemia-reperfusion in rats, via down-regulating the expression of ACE mRNA and inhibiting the activity of MPO. |
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