| Objective: The carcinoma of larynx is one of the most commom malignant neoplasms in otolaryngology and head and neck surgery, and the most commom pathology is the laryngeal squamous cell carcinoma comes frequently, whose morbidity increases gradually in recent years. New blood vessels and lymphatic vessels play a pivotal role in the processes of growth, infiltration and metastasis of laryngeal squamous cell carcinoma. VASH1 is an anti-angiogenesis and anti-lymphangiogensis regulatory factor which is found recently. Microvessel density and lymphatic vessel density are mostly utilized to evaluate the extent of angiogenesis and lymphangiogensis in tumor, respetively. In our study, the expression levels of VASH1, microvessel density(MVD) and lymphatic vessel density(LVD) in the laryngeal squamous cell carcinoma(LSCC) and adjacent tissue were detected to analyze the relationship between VASH1, MVD, LVD and the clinical pathological features, and the mutual relationship among VASH1, MVD and LVD. In addition, we investigated the role of VASH1 in the development and progression of laryngeal.Methods: 48 cases of LSCC and 23 cases of adjacent normal tissue from patients who were performed operation in our department from Mar. 2013 to Sep. 2014 were included in this study. The expression of VASH1, MVD(marked by CD34) and LVD(marked by CD34) in LSCC and specimens of adjacent tissue were detected by immunohistochemical staining. The relativitiy between the expression of VASH1 and MVD/LVD in LSCC were detected by double staining immunohistochemistry. Clinical data were analyzed with the SPSS 13.0. Enumeration data were compared using the chi-square test. Count data were summarized as mean±standard error of the mean, compared using the t-test. In the correlation analysis, Spearman-test was employed. In all studies, P values <0.05 were considered as statistically significant and the level of very significance was set at P<0.01.Results: 1 Immunohistochemical results 1.1 Using immunohistochemical staining to detect the expression of VASH1 in 48 cases of LSCC and 23 cases of adjacent normal tissue, the rate of positive expression were 43.8%(21/48) and 17.4%(4/23). There was significant difference in the positive rates of VASH1 protein between LSCC tissue and adjacent normal tissue(P<0.05). In LSCC tissue, the expression of VASH1 was correlated with lymph node metastasis, clinical stage and clinical classification. However, it wasn’t correlated with pathological grading of tumor and age. 1.2 Using immunohistochemical staining to detect the microvessel density in 48 cases of LSCC and 23 cases of adjacent normal tissue, there was very significant difference in microvessel density between LSCC tissue and adjacent normal tissue(33.34±8.29 vs. 16.85±5.21, P<0.01). In LSCC, microvessel density of the tissue was correlated with lymph node metastasis, clinical stage, clinical classification and pathological grading of tumor, but it wasn’t correlated with the age of patients. 1.3 Using immunohistochemical staining to detect the lymphatic vessel density in 48 cases of LSCC and 23 cases of adjacent normal tissue, there was very significant difference in lymphatic vessel density between LSCC tissue and adjacent normal tissue(9.64±3.52 vs. 5.82±2.33, P<0.01). In LSCC, the lymphatic vessel density of the tissue was correlated with lymph node metastasis, clinical stage, clinical classification and pathological grading of tumor, but it wasn’t correlated with the age of patients. 2 The mutual correlation among the expression of VASH1, MVD and LVDAnalysis of the experimental data showed that the expression of VASH1 and microvessel density were positively correlated(r=0.766, P<0.01) in LSCC tissue, and the expression of VASH1 and lymphatic vessel density were positively correlated(r=0.823, P<0.01). Positive correlation between the MVD and LVD was observed in our study(r=0.898, P<0.01).Conclusions:1 VASH1 was expressed in LSCC and specimens of adjacent tissue. Moreover, the positive expression rate in carcinoma tissue was higher than adjacent normal tissue. In LSCC tissue, the expression of VASH1 was correlated with lymph node metastasis, clinical stage, clinical classification. It may contribute to the growth, infiltration and metastasis of laryngeal squamous cell carcinoma tissue.2 The microvessel density and lymphatic vessel density in LSCC tissue were higher than adjacent normal tissue. In LSCC tissue, MVD and LVD were correlated with lymph node metastasis, clinical stage, clinical classification and pathological grading of tumor, indicating that MVD and LVD may be related to the growth, infiltration and metastasis of laryngeal squamous cell carcinoma tissue.3 In LSCC tissue, the expression of VASH1, MVD and LVD were positively correlated with each other, which indicated that VASH1 played a critical role in the process of angiogenesis and lymphangiogensis. |
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