CD86 Gene Polymorphisms Associated With Risk Of Colorectal Cancer

Objective:Colorectal cancer is one of the common malignant tumor in our country. Its incidence rate is rising year by year, and the exact mechanism is not very clear. The pathophysiological mechanisms of colorectal cancer is a process which related to a integrated interaction of multiple stages, multiple factors and multiple genes.Therefore,study of tumorrelated gene polymo- rphism may help us reveal the pathophysiological process of the color ectal cancer, and provide a new way for the diagnosis and treatment of colorectal cancer. At present, the study found that the small non coding RNA(micro RNA, mi RNA), whose single nucleotide polymorphism(single nucleotide micro RNA polymorphism, SNPs) is closely related to the occurrence and development of many malignant tumor, played an irreplaceable role in gene regulation and expression. Many studies about single nucleotide polymo- rphism and incidence rate of colorectal cancer were published abroad. For example, the gene polymorphism of CD86 in mi RNA was related to the incidence risk of malignant tumor such as pancreatic cancer and cervical cancer. Some research showed that gene polymorphism of CD86 had a relationship with the occurrence of colorectal cancer in people of Czech. However, it was not clear whether the gene polymorphism of CD86 was relevant to high incidence of colorectal cancer in Hebei population. Therefore, the structure and function, molecular mechanism of CD86 in mi RNA and its relationship with colorectal cancer were discussed in this study.Methods:Patients hospitalized by Fourth Hospital of Hebei Medical University from January, 2012 to January, 2015 in Hebei area were selected randomly into the study. patients and their families after informed consent They were divided into three groups: 102 cases of colon cancer group(CC group),138 cases of rectal cancer group(RC group) and 147 cases of normal control group(S group). Patients with other malignant tumors except colorectal cancer and precancerous lesions, congenital disease, mental disorders, abnormal coagulation function and puncture site infections were not included in the experiment. Age, gender, whether there is a history of alcohol or smoking and family history of patients were recorded. We collected 5ml of the peripheral venous blood in the objects of each group. Single genes and alleles typing in two gene polymorphism sites of Rs1129055G/A and rs17281995G/C in gene CD86 in peripheral blood were detected by using multiplex polymerase chain reaction- ligase detection reaction method.Experimental datas were analyzed statistically by using SPSS19.0 statistical software. genetic data of sex, whether there is a history of alcohol and family history were tested by χ2 test, data of age was analyzed by t test, data of genotype frequency of normal group objects was analyzed by the H-W equilibrium analysis, data of genes type and allele distribution data in colon cancer group, rectal cancer group and normal control group were analyzed by χ2 test, The Odds ratio and the 95% confidence interval of the risk degree were analyzed by non conditional Logistic regression analysis. Taking P=0.05 as the standard of test and P<0.05 as statistical significance.Results:1 Statistical analysis about population charcteristics in normal group.colon group and rectal cancer group. there was no statistical differrnce amomg normal group, colon cancer group and rectal caner group. Copared with normal group, there was no statistical difference in colon and rectal cancer group(P=0.036, P=0.038), It meaned that there are does relationship between incidence risk of colorectal cancer in Hebei area and family history.2 There was no statistical diference between rs 1129055G/A and rs 17281995 G/C in genotype frequency by Hardy-Weibery balacing text(P>0.05).It meaned that human sujects met law of genetic equilibriumand and the experiement was feasible.3 Analysis of incidence risk of colorectal cancer and gene polymorphism of rs 1229055G/A in CD86.Distribution frequency of G and A allele in G/A polymsrphism site of CD86 were 51.0%(104/204) and 49.0%(100/204) in colon cacer group, respectively 46.8%(131/280) and 53.2%(149/280) in rectal cacer group, respectively 59.9%(176/294) and 40.1%(118/294)in control group, repectively. Compared with control group, G/A genotype were different statistically between colon cancer group and rectal cancer group(P=0.02 and P=0.049).Distrbution frequency of GG/GA/AA alletic genes in rs1129055 G/A site of CD86 gene were 17.6%(18/102),66.7%(68/102) and 15.7%(16/102)in colon cancer group,respectively,16.4%(23/140),60.7%(85/140)and 22.9%(32/140) in rectal cancer group, respectively, 29.9%(44/147), 59.9%(88/147) and 10.2%(15/147) in control group. There was statistical difference between colon cancer group in GG/GA/AA genotype, and the GA genotype P value were 0.047 in colon cancer group and the AA genotype P value were 0.033 in colon cancer group and the GA genotype P value were 0.039 in rectal group and the AA genotype P value were 0.008 in rectal group.GA/AA genotype in colon and rectal cancer group.had a positive correlation with incidence rate of coloretal cancer in Hebei area.4 Correation analysis of incidence risk of coloretal cancer and gene polymorphism of rs 17281995G/C site in CD86.Distribution frequency of alletic genes in rs 17281995 G/C site of CD86 were 47.1%(96/204), 52.9%(108/204) in colon cancer group,respectvely 68.1%(188/276) and 31.9%(88/276) in rectal cancer group, respectively, 59.9%(176/294)and 40.1%(118/294)in control group, respectively, Cmpared with contrl group, there was statistical difference between colon cancer group and rectal cancer group in G/C monomer genotype.It meaned that G/C allelic gene in rs 17281995 G/C site of CD86 may increase the incidence rish of clorectal cancer in Hebei area.Pistribution fequency of GG/GC/CC genotype in 17281995 G/C site of CD86 were 29.4%(30/102), 35.3%(36/102) and 35.3%(36/102) in colon cancer group, respectively, 41.3%(57/138), 53.6%(74/138) and 5.1%(7/138) in retcal cancer group, repectively, 29.9%(44/147), 59.9%(88/147) and 10.2%(15/147) in control group, The GC genotype P value were 0.097 in colon cancer group and the CC genotype P value were 0.001 in colon cancer group, repectively, The GC genotype P value were 0.090 in retal cancer group and the CC genotype P value were 0.036 in retal cancer group, respectively. There was staistic difference in G/C genotype between colon cancer group and retal cancer group. It meaned that C/C genotype in 17281995 G/C site of CD86 may increase the incidence risk of coloretal cancer in Hebei area.Conclusion:1 SNP of 1129055G/A in CD86 may showed correlation with the incidence risk of colorectal cancer. The G/A genotype and A/A genotype may be associated with the incidence risk of colorectal cancer in Hebei area.2 SNP of rs17281995G/C in CD86 may showed correlation with the incidence risk of colon cancer. The C/C genotype may be associated with colorectal cancer incidence risk in Hebei area.