The Study Based On The Metabolic Pathway Of Intestinal Flora About The Mechanism Of Qiweibaizhu Powder In Treatment Of Infant With HRV Diarrhea

ObjectiveThe aim of this study was to observe the metabolic pathways of intestinal flora with function genomics analysis in pre/post-treatment, in order to investigate the mechanisms that Qiweibaizhu powder leads to its cure.MethodsMetagenomic sequencing results of gut microbiome in stool of RV infection children with pre/post-treatment were obtained through previous study. We assembled on genes and annotated the gene sets based on the KEGG database to find the functional genes participate in what pathways.ResultsA large number of microbial functional genes related to carbohydrate metabolism changed significantly after treatments. For example,the genes e ncoding the rate-limiting enzymes such as phosphoglucomutase(5.4.2.-2)、g lucose-6-phosphate isomerase(5.3.1.9)、6-phosphofructokinase(2.7.1.11)、 fructose-bisphosphate aldolase (4.2.1.13)、phosphoglycerate kinase(2.7.2. 3)、phosphoglycerate mutase(5.4.2.11)、phosphopyruvate hydratase(4.2.1. 11)、pyruvate kinase(2.7.1.40),were up-regulated, which may imply inc reased production of pyruvate.In Pyruvate metabolism,most of the genes en coding enzymes which involve in the creation of Acetic acid were up-regu lated,such as pyruvate formate-lyase(2.3.1.54)、dihydrolipoyl dehydrogen ase(1.8.1.4)、dihydrolipoyllysine-residue acetyltransferase(2.3.1.12)、 phosphate acetyltransferase (2.3.1.8)、acetate kinase(2.7.2.1).In the m etabolic pathway of propionate and butyrate, scenarios that genes up-regu lated and down-regulated were similar.There may be no significant increas e or decrease.ConclusionQiweibaizhu Powder may play a role in improving glucose metabolic pathways through modulating the intestinal flora. The production of pyruvate rased in glycolysis, affecting the creation of SCFAs,which could increase Na+-dependent fluid absorption,hence compensate water and electrolyte lost.