Different Formulations Qiweibaizhu Powder On Intestinal Flora In Mice With Diarrhea And The Treatment Of Immune-related Mechanism

Objective:The effects of different forms Qiweibaizhu powder on intestinal flora in mice therapeutic action and influence of the intestinal mucosal immunity.Method:1.Lincomycin hydrochloride injection with ceftriaxone injection combined with traditional Chinese Folium Sennae mice by gavage manufacturing diarrhea model intestinal flora.2.Groupsand treatment:After the successful model,mice were randomly divided into model group (MG)(then known as the natural recovery group ZFG), Qiweibaizhu Powder decoction group (QJG), Qiweibaizhu scattered drops group (QDG), Qiweibaizhu Powder extract group (QTG), and normal control group (NG). Each group began treatment in modeling fifth days, MG (ZFG) and NG given distilled water, the rest of the treatment group were given the appropriate dosage of the drug orally.3. Outcomemeasures:Giving the medicine to the first2days,4days,6days,8days to extract10per group, weighing, heart blood mice were killed, stool cultures observe the type and quantity of microorganisms; HE staining to observe of mouse intestinal pathology and observed with an electron microscope ultrastructure; Immu-nohistochemistry and RT-PCR method to detect intestinal epithelial IFN-a, IL-4,IL-10expressions.Results:1. After modeling in mice watery stool, stools rate, loose stools and diarrhea index level was significantly increased. The number of diarrhea during treatment of mice significantly reduced the rate of loose stools, loose stools and diarrhea index lower level, QJG, QDG and QTG compare with MG (p<0.05).2. Murine small intestine villi fracture model group, the epithelial cell swelling, partial necrosis, increased inflammatory cells. After giving the medicine4,6,8d each treatment group were significantly reduced intestinal mucosal pathological lesions, pathological scoring decline, QJG, QDG, QTG and ZFG statistically significant (p<0.05).3. Intestinal flora①after modeling MG (ZFG) mouse intestinal the feces Enterococci, Escherichia coli, Bifidobacterium,Lactobacillus number was significantly lower than the control group (p<0.05).②The treated mice intestinal fecal Bifidobacteria, Lactobacilli have begun to increase the total number in the give the medicine the2th days, give the medicine the8th days QJG, QDG and QTG two the total number of bacteria Compared with give the medicine the6th day,4th day,2th day was significantly higher p<0.05, compared with normal group p<0.05, three treatment groups Bifidobacteria, Lactobacilli significantly higher number in ZFG (P<0.05); QJG, QDG, QTG mouse intestinal fecal bifidobacteria, lactobacilli restore order QTG> QDG> QJG. With prolonged observation model group (ZFG) the total number of bifidobacteria and Lactobacilli mice also gradually restored, but with NG, QJG, QDG and QTG compare the difference is still significant (p <0.05).③M ouse intestinal fecal Enterococcus, E. coligive the medicine the2th days began to decline, QJG, QTG, QDG ZFG comparison with Enterococcus, Escherichia coli significantly decreased the number of p <0.05;8th day QTG coliform count was close NG (p>0.05), QJG, QDG also recover the rapid, but more (p<0.05) with the normal group. With the observation of prolonged ZFG mouse intestinal fecal enterococci, Escherichia coli number also declined, but compared with NG, QJG, QTG, QDG, the difference was statistically significant p<0.05.4. Giving the medicine to the2th days in each treatment group of mice began to gain weight, body weight of mice treated at different times were more important than the MG mice, the difference was statistically significant (p<0.05); body weight within the same group of mice comparing different treatment time The difference was statistically significant (p <0.05).5、①MG(ZFG) mouse intestinal IL-4expression was significantly lower than the NG (p<0.05), with prolonged expression was observed by nearly rise, but still amounted to less than give the medicine the8th days,NG, QJG, QTG, QDG levels (p<0.05); QJG, QTG, QDG mouse small intestinal mucosal expression of IL-4at each time point, the difference was not statistically significant (p>0.05); the three treatment groups increased expression of IL-4faster than ZFG, there are statistical differences significance (p<0.05).②IL-10expression levels of MG (ZFG) mouse intestinal tissue was significantly lower than NG (p<0.05), with the extension of ZFG IL-10expression was observed in mice by recent recovery time, but the observation to give the medicine the8th days, ZFG still not reached NG level of p<0.05, compared with QJG, QTG, QDG p<0.05. QJG, QTG, QDG murine IL-10expression increased faster than ZFG, the difference was statistically significant (p<0.05). Give the medicine the8th days, the small intestinal expression of IL-10NG difference compared to the three treatment groups were not statistically significant (p>0.05).③IFN-a expression in intestinal mucosa model mice was significantly higher than NG (p<0.05), higher than QJG, QTG, QDG p<0.05; with prolonged treatment time QJG, QTG, QDG mouse intestinal epithelial cells IFN-a expression by nearly fell, different treatment time comparison between the three groups, the difference was statistically significant (p<0.05), IFN-a expression at each time point were lower than ZFG (p<0.05), higher than the normal group, the difference was statistically significant (p<0.05).Conclusion:Different formulations were Powder Qiweibaizhu intestinal tract flora diarrhea in mice has a good therapeutic effect, can restore normal intestinal flora and protect the intestinal mucosa. The mechanism may be able to lowered loose Qiweibaizhu intestinal expression of IFN-a, increased IL-4, IL-10expression, thereby improving the intestinal immune environment, inhibit intestinal inflammation and pathological reactions.