Expression Of CEACAM6,FOXP3 And Their Influence On Pancreatic Cancer Occurrence,Development And Prognosis

Part I Expression of CEACAM6 and FOXP3 in pancreatic cancer and its effects on immune function and prognosisObjective This study aims to explore expression of CEACAM6,FOXP3 and infiltration density of tumor infiltrating lymphocyte cells(TILs) in pancreatic cancer, and their relationship with clinicopathological features and prognosis, also detect the relationship between CEACAM6, FOXP3 expression and infiltration density of TILs.Materials and Methods Forty cases of pancreatic cancer including 22 cases of stage I or II and 18 cases of stage III or IV were collected for this study from the First Affiliated Hospital of Soochow University between November 2006 and January 2014. The infiltration density of TILs, and the expression of CEACAM6 and FOXP3 were examined by immunohistochemical analysis. And to analyze their relationship with the clinicopathological features and prognosis.Results CD3+T, CD8+T, CD45RO+T cell infiltrations were significantly higher in stage I and II compared with stage III or IV, similarly, T cell infiltrations were higher in better pathological differentiation. Whereas, the expression of FOXP3 and CEACAM6 obviously increased in stage III or IV, and FOXP3 was higher in worse pathological differentiation. High FOXP3 expression was significantly associated with low CD3+T, CD8+T, CD45RO+T cell infiltrations(P=0.015, P=0.003, P=0.020, respectively). We further analyzed the relation between CEACAM6 expression and TILs infiltration, finding that high CEACAM6 expression associated with low CD8+T cell infiltration(P=0.001), and even low CD45RO+T cell infiltration(P=0.004). Patients with high expression CEACAM6 and FOXP3 survival period is shorter.Conclusion Expression of CEACAM6 and FOXP3 in pancreatic cancer patients plays an important role in the inhibition of T cell response, and is closely related to the prognosis of tumor. Part II CEACAM6,FOXP3 and immune function changes impact on theoccurrence and development of pancreatic cancerObjective To study the changes of CEACAM6,FOXP3 and TILs in chronic pancreatitis and pancreatic cancer.And to discuss their impact on the occurrence and development of pancreatic cancer.Materials and Methods Sixty cases of pancreatic tissues including twenty-two stage I or II and eighteen stage III or IV, twenty cases of chronic pancreatitis were collected for this study from the First Affiliated Hospital of Soochow University between November 2006 and January 2014. The infiltration density of TILs,and the expression of CEACAM6 and FOXP3 in pancreatic tissues were examined by immunohistochemical analysis. Development of a Real-time PCR assay based on SYBR Green I for detection of CD3+T, CD4+T, CD8+T and CD45RO+T cell,CEACAM6 and FOXP3 m RNA levels in paraffin block specimen.And analysis the relationship with the clinical pathological characteristics and pancreatic cancer progression.Results CD3+T, CD8+T and CD45RO+T cell infiltrations in stage I or II and III or IV were lower than in chronic pancreatitis(P<0.05), especially lower in stage III or IV compared with stage I or II(P<0.05). The expression of CEACAM6 and FOXP3 in stage I or II and III or IV were higher than in chronic pancreatitis(P<0.05),especially higher in stage III or IV compared with stage I or II(P<0.05).Results verification by the method of Realtime PCR and immunohistochemical anlysis are basically identical.Conclusion CEACAM6,FOXP3 and TILs are significantly associated with the occurrence and development of pancreatic cancer. Immune reaction gradually varies between different stages of pancreatic cancer processes.