Scorpon Spasmolysis Cure Huang Xiao Granule On Asthma Mice P38Lightning MAPK And Th1/Th2Cytokines Changes

Objective: Bronchial asthma is xiao [1], out of the category of ancient Chinese medicine.The nosogenesis of asthma is sophisticated. Three traditional Chinese medicine is generallybelieved that the lung, spleen and kidney dysfunction and induced asthma is closely related tothe hair, three dirty adjust the normal operation of the body fluid metabolism, when threedysfunction, get wet phlegm, stagnation in the lung, become the potential controlling asthma"roots"rewelding[2].Modern pathology study that bronchial asthma is by a variety ofinflammatory cells(such as eosinophils and neutrophils, macrophages, lymphocytes, and so on)and inflammatory medium common contributing to the chronic allergic reaction caused theoccurrence of asthma.This topic proposed by establishing the attacks of asthma animal modelsin mice and explore the scorpion spasmolysis cure huang xiao particles adjust asthma airwayinflammation and airway fibrosis mechanism, has provided the new mentality for themechanism of airway remodeling of asthma, and also for the prevention and control of asthmadrug development to provide effective theory basis.Methods:6-8yearling to24male mice were randomly divided into four groups: normalcontrol group, the asthma model group, the scorpion spasmolysis cure huang xiao particleswere the low-dose group, spasmolysis cure huang xiao granule high-dose group.Out ofnormal control group, the asthma model group, the scorpion spasmolysis cure huang xiaoparticles were the low-dose group and spasmolysis cure huang xiao granule high-dose groupevery10including mice celiac injection of soluble in PBS gOVA sensitization and aluminumhydroxide mixture0.5ml.And on the21st day at the same time every day for3consecutivedays proocation tests in mice, the atomization inhalation,every time30minutes preparation ofairway inflammation of asthma model in mice.Both groups of mice respectively quantitativegive corresponding experimental drugs regularly.Records of the total quantity of inflammationcells in BALF groups of mice, classification and gauge array; Changes of inflammatorycorpuscle in BALF; The pathological changes of lung tissue; Changes in airway epithelialcells; Inflammatory cytokines in mice BALF IL-4, IL-5, IFN-gamma content changes;The expression of phosphorylated p38MAPK protein in lung tissue.Analysis and contrastbefore and after treatment the observation if there is a test. Results:Scorpion spasmolysis cure huang xiao granule can effectively reduce asthmaairway inflammatory cells and inflammatory factors; The content of inflammatory factorsIFN-γ increased,Decreasing the content of IL-4, IL-5;Improve the air passage of epithelialcells, produce inflammation; Lung tissue expression prevents the phosphorylation ofp38MAPK protein, Transmission blocking nuclear signal.Through the experimental resultsconfirm the scorpion spasmolysis cure huang xiao granule can repair the damaged lung tissue,reduce airway inflammation, airway fibrosis and airway remodeling, availability depress therecurernce rate of asthma.Conclusion: Scorpion spasmolysis cure huang xiao granule could inhibit asthma micephosphorylated p38lightning MAPK protein expression and regulation of the role of Th1/Th2balance.